[Assessing abundance and specificity of different types of sgRNA targeting miRNA precursors].

CRISPR/Cas technology enables efficient and specific editing the genome. Since different bacterial sources or artificially modified Cas9, as well as Cpf1 and other nucleases, recognize different PAMs (protospacer adjacent motifs), different gene editing nucleases may use different types of sgRNAs (small guide RNA). MicroRNAs (miRNAs) are a class of regulatory small non-coding RNAs.

[sgRNA design for the CRISPR/Cas9 system and evaluation of its off-target effects].

The third generation of CRISPR/Cas9-mediated genome editing technology has been successfully applied to genome modification of various species including animals, plants and microorganisms. How to improve the efficiency of CRISPR/Cas9 genome editing and reduce its off-target effects has been extensively explored in this field. Using sgRNA (Small guide RNA) with high efficiency and specificity is one of the critical factors for successful genome editing.

Development of a graphical user interface for sgRNAcas9 and its application.

The CRISPR/Cas9 genome editing technique is a powerful tool for researchers. However, off-target effects of the Cas9 nuclease activity is a recurrent concern of the CRISPR system. Thus, designing sgRNA (single guide RNA) with minimal off-target effects is very important.

[Genotype of isolated strain of hantavirus in Liaoning area].

Objective: To isolate hantavirus and to genotype isolated strains of hantavirus (SY 13) in Liaoning province.

Methods: Hantavirus was isolated by means of cell culture. The G1, G2 fragments of M segments and S segment of the strain of SY 13 were amplified respectively by means of RT-PCR in order to compare and analyze the homology between the sequences of the three isolated gene fragments and the sequences downloaded from GenBank with neighbor-joining through sequence analysis software of DNA Star, Clustal, Phylip, etc.