[Retrospective study on transcrestal sinus floor elevation with simultaneous implantation of short implants].

Objective: To explore the changes in bone height of the maxillary sinus floor at different sinus ridge heights after transcrestal sinus floor elevation (tSFE) with the simultaneous implantation of short implants.

Methods: A total of 74 Bicon short implants were implanted into 37 patients during the same period of maxillary sinus elevation. The residual bone height (RBH)<4 mm group has 43 sites, and the RBH≥4 mm group has 31 sites.

[Studies on chemical constituents from roots of Caragana sinica].

Objective: To study the chemical constituents in roots of Caragana sinica.

Method: The constituents were isolated by silica gel column chromatography, and their structures were identified by spectroscopic methods.

Result: Seven compounds were identified as (+) - stenophyllol B (1), 4-hydroxybenzoic acid (2), odoratin (3), resveratrol (4), cararosinol A (5), leachinol C (6), carasinaurone (7) respectively.

(4S,8S,9R,12E)-8,9,16,18-Tetrahydroxy-4-methyl-3-oxabicyclo[12.4.0]octadeca-12,14,16,18-tetraen-2-one monohydrate.

The asymmetric unit of the title compound, C(18)H(24)O(6)·H(2)O, contains a 14-membered macrolide mol-ecule and a water mol-ecule. In the crystal structure, intra-molecular C-H⋯O and O-H⋯O hydrogen bonds help to stabilize the mol-ecular conformation, while inter-molecular O-H⋯O hydrogen bonds link the mol-ecules, forming an infinite network. The absolute configuration was assigned by comparison with related zearalenone compounds, but needs verification.

[Anti-HIV chemical constituents of aerial parts of Caragana rosea].

This study was intended to look for anti-HIV chemical constituents of aerial parts of Caragana rosea Turcz. Column chromatographic technique was used for the isolation and purification of constituents of Caragana rosea under the guide of anti-HIV assay. The structures were established on the basis of physical and chemical properties and spectroscopic data.

[Studies on the chemical constituents from Caragana intermedia].

Objective: To study the chemical constituents from Caragana intermedia.

Methods: The compounds were separated by chromatography methods. Their structures were identified by spectral analysis.

Hypericum sampsonii induces apoptosis and nuclear export of retinoid X receptor-alpha.

Natural products derived from plants provide a rich source for development of new anticancer drugs. Recent studies suggest that modulation of subcellular localization of retinoid X receptor-alpha (RXRalpha) represents a potential approach for inducing cancer cell apoptosis. In this study, we screened a herbal library for inducing translocation of RXRalpha from the nucleus to the cytoplasm.

Metabolites and the pharmacokinetics of kobophenol A from Caragana sinica in rats.

This research aims to study the metabolism and pharmacokinetics of phytoestrogen kobophenol A (1), the main active compound of Caragana sinica (Buc'hoz) Rehd. (Fabaceae), in rats. Metabolites of 1 in rats' feces were isolated and purified by multi-chromatograph techniques; three new metabolites of 1, named koboquinone A (M1), koboquinone B (M2) and koboquinone C (M3), were isolated, purified from rats' feces after they being orally administered with 1.

Anti-HIV bioactive stilbene dimers of Caragana rosea.

Bioassay-guided fractionation of the ethanol extract of the aerial parts of Caragana rosea Turcz. led to the isolation of two new (cararosinol C and cararosinol D) and four known [scirpusin A (1), cis-scirpusin A (2), maackin (3), scirpusin B (4)] stilbene dimers. This is the first identification of these compounds from this plant and the first time that compound 2 has been isolated as a natural compound.

Two new oligostilbenes from Caragana sinica.

A new resveratrol dimer, carasiphenol A (1), and a new resveratrol trimer, carasiphenol B (2), have been isolated from the aerial parts of Caragana sinica. Their structures have been elucidated from spectroscopic evidence, especially HMBC and NOE experiments. The relative configuration of the known dimer pallidol (6) was confirmed by X-ray diffraction.

[Studies on flavonoid constituents of Caragana intermediia].

Aim: To study the chemical constituents of Caragana intermedia.

Methods: The compounds were separated by chromatography methods, their structures were identified by spectral analysis.

Results: Ten compounds were isolated and identified as 5,7,4'-trihydroxy-3,3'-dimethoxyflavone (1), 3,5,7,8,4'-pentahydroxy-3'-methoxyflavone(2), puercetin(3), limocitrin(4), 5,7,3',4'-tetrahydroxy-3-methoxyflavone(5), 7,3',5'-trihydroxyflavanone(6), 5,7,3',4'-tetrahydroxy-3,8-dimethoxyflavone(7), butein(8), liquiritigenin(9) and 5,7,4'-trihydroxy-3,8-dimethoxyflavone(10).

Constituents from the stems of Goniothalamus griffithii.

A new cyclopeptide, grifficyclocin A (1), and a new aporphine alkaloid, griffinin (2) were isolated together with two known styryllactones from the stems of Goniothalamus griffithii. Their structures were identified spectroscopically and chemically. Among them, the griffinin (2) was isolated as the enol form in two tautomers, and the two known styryllactones, goniothalamin (3) and 8- O-acetylgoniotriol (4), showed selective in vitro antitumor activities.

[The binding sites of estrogen receptor for miyabenol C and kobophenol A].

To examine the binding sites of miyabenol C (Miy C) and kobophenol A ( Kob A) with estrogen receptor (ER), computer modeling was applied to determine 3D structure of Miy C and Kob A. Molecular docking of the components to ER was carried out to find the binding sites between them. PCR mutagenesis was used to change the structure of ER cDNA.

Assessment of estrogenic activity of natural compounds using improved E-screen assay.

Aim: To improve E-screen assay and make it more accurate to screen estrogenic compounds.

Methods: Estrogen receptor antisense RNA expression plasmid (pCASER) was constructed and introduced into MCF-7 with lipofectAMINE(TM), and positive clones were screened out with G418. PCR amplification was employed to identify whether estrogen receptor (ER) cDNA fragment had been inserted into MCF-7 cell genomes.