Simultaneous detection of human norovirus GI, GII and SARS-CoV-2 by a quantitative one-step triplex RT-qPCR.

Background: There are many similarities in the clinical manifestations of human norovirus and SARS-CoV-2 infections, and nucleic acid detection is the gold standard for diagnosing both diseases. In order to expedite the identification of norovirus and SARS-CoV-2, a quantitative one-step triplex reverse transcription PCR (RT-qPCR) method was designed in this paper.

Methods: A one-step triplex RT-qPCR assay was developed for simultaneous detection and differentiation of human norovirus GI (NoV-GI), GII (NoV-GII) and SARS-CoV-2 from fecal specimens.

A novel "off-on" fluorescent probe for the detection of nickel ions and its clinical application.

Nickel serves as an essential micronutrient for the human body, playing a vital role in various enzymatic processes. However, excessive nickel entering the environment can cause pollution and pose serious risks to animals, plants, and human health. High concentrations of nickel ions in the human body increase the risk of various diseases, highlighting the need for accurate measurement of nickel ions levels.

The retracted conformation of ubiquitin Ser65 phosphorylation inhibits the formation of K48-linked ubiquitin chains.

PINK1, as the first reported ubiquitin kinase, can phosphorylate ubiquitin (Ub) at Ser65 site, which regulates the structure and function of Ub monomer. However, the levels of PINK1 and phosphorylated Ub (pUb) are very low in normal cells. Here we show that when proteasome activity is inhibited, the levels of soluble PINK1 (sPINK1) and pUb will increase significantly.

Ubiquitin S65 phosphorylation engenders a pH-sensitive conformational switch.

Ubiquitin (Ub) is an important signaling protein. Recent studies have shown that Ub can be enzymatically phosphorylated at S65, and that the resulting pUb exhibits two conformational states-a relaxed state and a retracted state. However, crystallization efforts have yielded only the structure for the relaxed state, which was found similar to that of unmodified Ub.

Quinoline-based molecular clips for selective fluorescent detection of Zn2+.

New selective Zn2+ fluorescent sensors, di(2-quinoline-carbaldehyde)-2,2'-bibenzoyl-hydrazone (QB1) and di(2-quinolinecarbaldehyde)-6,6'-dicarboxylic acid hydrazone-2,2'-bipyridine (QB2), have been designed and prepared. Both QB sensors exhibit an emission band centered at 405 nm (excitation at 350 nm) with low quantum yield. Zinc binding not only red-shifts the emission band to 500 nm, but also enhances the fluorescence intensity by an order of magnitude based on the deprotonization strategy via self-assembly.