[Spatio-temporal Characteristics of Organic Aggregates and the Driving Factors in Typical Lakes].

Organic aggregates (OA) are the important circulation hub of matter and energy in aquatic ecosystems. However, the comparison studies on OA in lakes with different nutrient levels are limited. In this study, spatio-temporal abundances of OA and OA-attached bacteria (OAB) in oligotrophic Lake Fuxian, mesotrophic Lake Tianmu, middle-eutrophic Lake Taihu, and hyper-eutrophic Lake Xingyun were investigated in different seasons during 2019-2021 using a scanning electron microscope, epi-fluorescence microscope, and flow cytometry.

[Sources and pollution characteristics of antibiotic resistance genes and conditional pathogenic bacteria in concentrated swine feeding operation.].

Air in concentrated animal feeding operations contains antibiotic resistance genes and airborne pathogens, with potential threat to human and animal health. In this study, air was sampled in the living area, outside, and inside of a fattening pig house in a pig farm for 24 and 48 hours. Feedstuffs, drinking water additives, and feces in the pig house were collected.

[Study on correlation between trace elements and active ingredient in glycyrrhizae radix et rhizome].

To clear the kinds of trace elements which is closely related with the active ingredient, proclaim the effects of trace elements on the quality of the Glycyrrhizae Radix et Rhizoma, provide the theoretical foundation to the further quality control of cultivation, take the advantage of the HPLC to determine the contents of glycyrrhizic acid and the liquiritin according to Chinese pharmacopoeia, use the inductively coupled plasma mass spectrometry to test the contents of Cu, Zn, Mn, Pb, Se, Cd, Ni, La, Na, Cr, M, Fe, Ca, Al, K, Sr, then, use SPSS statistical software for active ingredient and trace elements Correlation Analysis. The result of correlation analysis showed that Liquiritin contents of Glycyrrhizae Radix et Rhizoma have strong a positive correlation with the Mn, Pb contents, well, have a negative correlation with the Cu, Na contents; Glycyrrhizic acid contents showed a positive correlation with Mg, Cd, La contents, however, it showed a negative correlation with K, Fe contents. Comprehensive analysis of the results of the study, a preliminary thought that the active ingredient of Glycyrrhizae Radix et Rhizoma closely related with the trace elements, but the exact conclusion still need further study concentration-response relationship analysis.

[Study on the pollen viability and stigma receptivity of Chrysanthemum morifolium 'Fubaiju'].

Objective: To study the blooming habits, pollen viability and stigma receptivity of Chrysanthemum morifolium and provide theoretical basis for its breeding.

Methods: Explored the blooming habits by dynamic observation on the process of blossom, evaluated the pollen viability by germination in vitro culture method and estimated stigma receptivity by benzidine-hydrogen peroxide method.

Results: About the pollen viability, there were no significant differences between the flowers which in the same round of the capitulum; Tubular flowers in the center of a capitulum were significantly higher than that on the edge; In the morning pollen vitality gradually raised, during 11: 00 - 14: 00 maintained the highest, and then gradually decreased; Tubular flower began to loose powder on the third day, during 4th - 6th day the pollen viability was highest, respectively was 35.

[Research advances of genomic GYP coding MNS blood group antigens].

The MNS blood group system includes more than 40 antigens, and the M, N, S and s antigens are the most significant ones in the system. The antigenic determinants of M and N antigens lie on the top of GPA on the surface of red blood cells, while the antigenic determinants of S and s antigens lie on the top of GPB on the surface of red blood cells. The GYPA gene coding GPA and the GYPB gene coding GPB locate at the longarm of chromosome 4 and display 95% homologus sequence, meanwhile both genes locate closely to GYPE gene that did not express product.

[Screening and characteristics of normal temperature lignocellulose-degradation microbial community].

A normal temperature lignocellulose-degrading microflora has been constructed by our laboratory. We researched the degradation activity and compose of the community in 28 degrees C fermentation condition. The results showed that the microbial community could degrade 39.

[Composition diversity of the multifunctional bacterium community NSC-7].

The NSC-7 microbial community could decompose cellulose and lindan with high efficiency. In order to determine the bacterial composition of the community, 11 isolate strains were detected by plate isolation, while a community reset by the 11 isolate strains lost the capacity of degrading cellulose. The capacity of degrading of the filter paper in double deck plate and monolayer plate were determined, only the filter paper in double deck plate were degraded, that means the main or key microbe are anaerobic.

Evaluation of the performance of the EIAgen HCV test for detection of hepatitis C virus infection.

The EIAgen HCV test (Adaltis Inc., Montreal, Canada) is an enzyme immunoassay (EIA) for the detection of anti-hepatitis C virus (HCV) antibodies. This study compared the performance of this test side-by-side with the current Ortho HCV 3.

[Application of microfluidic chip analytical systems in ABO genotyping].

Limitations of polyacrylamide gel or agarose gel electrophoretic methods in genotyping research affect the interpreting of detection results. In order to develop a simple and reliable method for appraising results of ABO genotyping detection, the microfluidic chip analysis system was established by using microfluidic chip to replace the gel electrophoresis and combining with multiplex-PCR-RFLP technique. 150 blood samples were tested by this microfluidic chip analysis system with multiplex-PCR-RFLP technique to evaluate its stability and accuracy.

[Impact of glyphosate and acetochlor on Dugesia japonica ingestion and regeneration].

In this paper, the acute toxicity of glyphosate and acetochlor on Dugesia japonica and the impact of these two chemicals on the ingestion and regeneration of D. japonica were studied. The results showed that the 24 h and 48 h LC50 of glyphosate and acetochlor on D.

[Study on geographical variation of morphologic and germination characteristic of different Glycyrrhiza uralensis provenance seeds].

Objective: To study the geographical variation of morphologic and germination characteristic of different Glycyrrhiza uralensis provenance seeds, approach the geographical variation mode and ecology mechanism, and laid theoretical foundation for districting and allocating of G. uralensis seeds.

Method: Field investigation and laboratory analysis were applied.

[Productions analyses and pH dynamics during rice straw degradation by the lignocellulose degradation bacteria system WSC-6].

To detect the metabolic characteristic of rice straw degradation by composite microbial system WSC-6, we cultured WSC-6 in the media used rice straw as the limiting carbon source. The rice straw was added in the style of different quantity once or the same quantity at the different time intervals during 90 days culture. The systems were cultivated under static condition at 50 degrees C.

[ABO genotyping of Han population in Beijing].

The aim of this study was to establish a diagnostic method for ABO genotyping and to investigate the distribution of ABO genotype in Beijing Han population so as to understand the distribution characteristics and regularity of ABO genotype. An ABO genotyping method was established by using multiplex-PCR-RFLP and PCR-SSP techniques, and the ABO allele frequency in Beijing Han population was investigated. The results showed that A102, O1 and B allele were more common genes in Beijing Han individuals.

[Microbial dynamics during the composting process].

Microbial dynamics of microbial community during the composting process was investigated with the methods of denaturing gradient gel electrophoresis (DGGE) and plating. The results showed that the most of microorganism count was bacterium, the second was actinomycete and the least was fungi. At the same time, the count of the thermophilic microorganism was always less than that of the mesophilic during the composting process.

Bile tract adenomyoma: a case report.

This paper described a rare case of adenomyoma of common bile duct. The case is a 51-year-old man who was hospitalized for yellow color skin and sclera and itching for 2 mo without abdominal pain. Nothing special was found in physical examination except yellowish skin and sclera.

A multi-Chinese blood center study testing serologic-negative donor samples for hepatitis C virus and human immunodeficiency virus with nucleic acid testing.

Background: A multi-blood center study was conducted to evaluate a human immunodeficiency virus type 1 (HIV-1) and hepatitis C virus (HCV) multiplex nucleic acid testing (NAT) donor screening test and to determine the residual risk for HIV-1 and HCV infection.

Study Design And Methods: A commercially available HIV-1 and HCV assay (Procleix, Chiron Corp.) was used for simultaneous detection of HIV-1 RNA and HCV RNA on 89,647 unlinked donor samples.

[Review on molecular regulation of in vitro shoot regeneration of plants].

In vitro shoot regeneration is a developmental process that multiple genes and their interactions are involved in. Initiation of somatic cells stimulated by cytokinin, division of the initiated somatic cells and succeeding shoot meristem development are three key steps in the process. Therefore, exploration of gene expression and their interactions involved in these steps will shed light on our understanding of the molecular mechanism for regulation of plant in vitro shoot regeneration.

[Stability of hepatitis C virus RNA in various processing and storage conditions].

The study was purposed to investigate whether processing and storage conditions might influence the stability of the HCV RNA in whole blood or in plasma. The samples obtained from seven patients known to be positive for HCV RNA were kept in different storage conditions with different anticoagulants, and at the end of processing the plasma samples were frozen at -80 degrees C until fluorescent quantitative PCR testing. The results showed that there was no significant loss of HCV RNA titers in whole blood anticoagulated with CPDA or ACD or EDTA or none (P > 0.

Significance of the signal-to-cutoff ratios of anti-hepatitis C virus enzyme immunoassays in screening of Chinese blood donors.

Background: The correlation between signal-to-cutoff (S/CO) ratios of a second-generation hepatitis C virus (HCV) enzyme immunoassay (EIA; Abbott) and a third-generation HCV enzyme-linked immunosorbent assay (ELISA; Ortho) and confirmed HCV infection has been reported. The utility of the values for the Chinese anti-HCV EIA kits, however, has not been studied in evaluating test results in Chinese blood donors.

Study Design And Methods: A total of 156 donor samples repeat reactive for anti-HCV at routine screening from five representative regions of China were retested for anti-HCV by the Ortho third-generation HCV ELISA and six Chinese EIA kits and for HCV RNA by a human immunodeficiency virus-1 and HCV assay (Procleix, Chiron Corp.

[Correlation between signal/cutoff ratios of anti-HCV enzyme immunoassay (EIA) and their true positivity in blood donors].

Objectives: To evaluate the correlation between signal/cutoff (S/CO) ratios of anti-HCV EIA and their true positivity for determining the predictive value of S/CO ratios.

Methods: One hundred and fifty-nine samples of blood from donors positive for anti-HCV at the initial screening were collected from Beijing, Guangzhou, Hangzhou, Kunming and Urumchi. All the samples were retested by Ortho and 6 Chinese domestic anti-HCV EIA kits in duplicate, and detected for HCV RNA (NAT) using Chiron Procleix HIV/HCV system (transcription mediated amplification, TMA).

[The underground part growth distribution pattern of Glycyrrhiza uralensis and its effects on glycyrrhizinic acid content].

Objective: To ascertain the relationship between glycyrrhizinic acid content and the underground part growth character of Glycyrrhiza uralensis and provide the theoretical evidence for wild resources protection and artificial cultivation method of G. uralensis.

Method: Through the analytical investigation on the underground part of G.

[Effect of drought stress on growth of Glycyrrhiza uralensis].

Objective: To study the effect of drought stress on the growth of Glycyrrhiza uralensis and its drought resistance characteristic.

Method: The growth in dicators of G. uralensis including leaf, stem, root and biomass were measured when two-years-old G.

[The associations of HLA-DQB1 gene with onset age and autoantibodies in type 1 diabetes].

Objective: To evaluate the associations of human leukocyte antigen (HLA) DQB1 gene with onset age and autoantibodies in type 1 diabetes mellitus(T1DM) in Chinese Han population in Sichuan area.

Methods: Forty-six type 1 diabetic patients and 52 healthy control subjects were involved in this study. HLA-DQB1 typing was performed by polymerase chain reaction-sequence specific primer(PCR-SSP).