Imipenem exposure influences the expression of quorum-sensing receptor sdiA in Escherichia coli.

The increasing trend of carbapenem resistance amongst Escherichia coli poses a major public health crisis and requires active surveillance of resistance mechanisms to control the threat. Quorum-sensing system plays a role in bacterial resistance to antibiotics. Quorum sensing is a cell-cell communication system where bacteria alter their gene expression in response to specific stimuli.

Imipenem and meropenem influence the Las/Rhl quorum-sensing systems in clinical isolates of Pseudomonas aeruginosa.

The present study was conducted to study the influence of imipenem and meropenem at subinhibitory concentration on the transcriptional response of Las/Rhl quorum-sensing systems in isolates of Pseudomonas aeruginosa. In the present study, six representative carbapenem nonsusceptible clinical isolates of P. aeruginosa were obtained.

Tn5406, a staphylococcal transposon associated with macrolide-lincosamide-streptogramin resistance in clinical isolates of Staphylococcus aureus.

Purpose: In this study, we aimed to investigate the occurrence of MLS resistance in clinical isolates of Staphylococcus aureus with respect to their association with transposons.

Methods: The present study was performed with clinical isolates of S. aureus.

Transcriptional expression of secondary resistance genes ccdB and repA2 is enhanced in presence of cephalosporin and carbapenem in Escherichia coli.

Background: The issue of carbapenem resistance in E.coli is very concerning and it is speculated that cumulative effect of both primary resistance genes and secondary resistance genes that act as helper to the primary resistance genes are the reason behind their aggravation. Therefore, here we attempted to find the role of two secondary resistance genes (SRG) ccdB and repA2 in carbapenem resistance in E.

Use of fluorescence foldscope as an effective tool for detection of biofilm formation in .

Purpose: Pseudomonas aeruginosa is an opportunistic pathogen with biofilm-forming ability, by the virtue of which they can evade the immune response and antimicrobial chemotherapy. Several methods have been designed for the detection of biofilms but require sophisticated instrumentation and expertise. The present study, therefore, used an improvised device, 'fluorescence foldscope' which is an origami-based fluorescence microscope as an easy and effective tool to detect biofilm formation.

Escherichia coli encoding bla associated with community-acquired urinary tract infections with unusual MIC creep-like phenomenon against imipenem.

Objectives: Carbapenemase-producing Escherichia coli are a major clinical concern. The current study aimed to identify NDM-5-producing E. coli associated with community-acquired urinary tract infections (UTIs) co-harbouring extended-spectrum β-lactamases (ESBLs) and showing a phenomenon of imipenem minimum inhibitory concentration (MIC) creep.

Occurrence of bla on an IncI1 plasmid in multidrug-resistant Escherichia coli from a tertiary referral hospital in India.

Objectives: Plasmids of different replicon types are believed to be associated with the carriage and transmission of antimicrobial resistance genes. The present study was undertaken to examine the association of bla with particular plasmid types and to identify Escherichia coli strains involve in the maintenance of this resistance determinant in the plasmid.

Methods: Phenotypic screening of AmpC β-lactamases was performed by the modified three-dimensional extract method, followed by antimicrobial susceptibility testing and determination of minimum inhibitory concentrations (MICs).