RP-HPLC-RID analysis of InsP to InsP in Indian cereals, legumes, and their products: A comparative evaluation of PRP-1 Vs C18 column.

Phytic acid or inositol hexakisphosphate (InsP) and its dephosphorylated forms (InsP, InsP & InsP) are integral to cellular functions and confer several health benefits. The present study was aimed to develop a cost effective and high sample throughput RP-HPLC-RID method for routine quantification of lower inositol phosphates in both raw and processed cereals and pulses. For this asuitable mobile phase composition was formulated and two columns (MacroporusHamilton PRP-1 Vs Waters Symmetry C18) were compared in terms ofsystem specificity,linearity, accuracy and precision.