Immunomodulatory activity of aqueous extract of flowers with particular reference to splenocytes proliferation and cytokines induction.

Objectives: To investigate the immunomodulatory activity of aqueous extract of flowers (NAFE) with particular reference to splenocytes proliferation and induction of cytokines.

Materials And Methods: Antibody titer was determined by tube agglutination and indirect ELISA assay in four groups of mice-control, antigen alone, and NAFE-treated (400 and 800 mg/kg for 21 days) after immunization with antigen while cellular immunity was studied in three groups of rats (control and NAFE-treated - 400 and 800 mg/kg) following DNCB application. Splenocytes from untreated and NAFE-treated rats were stimulated using concanavalin-A (Con-A) and optical density (OD) and stimulation index were determined.