Seeking arrangements: cell contact as a cleavage-stage biomarker.

Research Question: What can three-dimensional cell contact networks tell us about the developmental potential of cleavage-stage human embryos?

Design: This pilot study was a retrospective analysis of two Embryoscope imaging datasets from two clinics. An artificial intelligence system was used to reconstruct the three-dimensional structure of embryos from 11-plane focal stacks. Networks of cell contacts were extracted from the resulting embryo three-dimensional models and each embryo's mean contacts per cell was computed.

Predicting the number of oocytes retrieved from controlled ovarian hyperstimulation with machine learning.

Study Question: Can machine learning predict the number of oocytes retrieved from controlled ovarian hyperstimulation (COH)?

Summary Answer: Three machine-learning models were successfully trained to predict the number of oocytes retrieved from COH.

What Is Known Already: A number of previous studies have identified and built predictive models on factors that influence the number of oocytes retrieved during COH. Many of these studies are, however, limited in the fact that they only consider a small number of variables in isolation.

Machine learning for prediction of euploidy in human embryos: in search of the best-performing model and predictive features.

Objective: To assess the best-performing machine learning (ML) model and features to predict euploidy in human embryos.

Design: Retrospective cohort analysis.

Setting: Department for reproductive medicine in a university hospital.

Predictive modeling in reproductive medicine: Where will the future of artificial intelligence research take us?

Artificial intelligence (AI) systems have been proposed for reproductive medicine since 1997. Although AI is the main driver of emergent technologies in reproduction, such as robotics, Big Data, and internet of things, it will continue to be the engine for technological innovation for the foreseeable future. What does the future of AI research look like?

Data sharing: using blockchain and decentralized data technologies to unlock the potential of artificial intelligence: What can assisted reproduction learn from other areas of medicine?

The extension of blockchain use for nonfinancial domains has revealed opportunities to the health care sector that answer the need for efficient and effective data and information exchanges in a secure and transparent manner. Blockchain is relatively novel in health care and particularly for data analytics, although there are examples of improvements achieved. We provide a systematic review of blockchain uses within the health care industry, with a particular focus on the in vitro fertilization (IVF) field.

The minimal gene set member msrA, encoding peptide methionine sulfoxide reductase, is a virulence determinant of the plant pathogen Erwinia chrysanthemi.

Peptide methionine sulfoxide reductase (MsrA), which repairs oxidized proteins, is present in most living organisms, and the cognate structural gene belongs to the so-called minimum gene set [Mushegian, A. R. & Koonin, E.

Nucleotide sequence and molecular characterization of pnlA, the structural gene for damage-inducible pectin lyase of Erwinia carotovora subsp. carotovora 71.

In a previous study, pnlA (the DNA damage-inducible structural gene for pectin lyase) of Erwinia carotovora subsp. carotovora 71 was localized to a 1.4-kb DNA segment within a 3.

Characterization of a new endoglucanase from Erwinia chrysanthemi.

The structural gene coding for a new endo-beta-1,4-glucanase of Erwinia chrysanthemi strain 3665, previously identified in a cosmid library, was subcloned into pUC18. The gene is expressed from a 1.9 X 10(3)-base-pair insert and its direction of transcription was determined.

Mutants of Erwinia chrysanthemi defective in secretion of pectinase and cellulase.

Erwinia chrysanthemi produced several pectate lyases (EC 4.2.2.

Cellobiose metabolism in Erwinia: genetic study.

The study of mutants of Erwinia specifically unable to ferment cellobiose indicates that the mutations are clustered between arg and ile on the chromosome of this organism. In vivo cloning of the genes responsible for cellobiose utilization lead to a plasmid, pBEC2, which complements all Erwinia Clb- specific mutants. When introduced into wild-type E.

The use of 6-labeled glucose to assess futile cycling in Escherichia coli.

To assess the "futile cycle" fructose-6-P leads to fructose-1,6-P2 leads to fructose-6-P in Escherichia coli we have grown the cells on [6-14C]glucose and determined label in the 1-position of glucose obtained from glycogen. In a variety of strains, including a wild type and a mutant without fructose diphosphatase, 1-position labeling was negligible. But there was little label in the 1-position of fructose-1,6-P2 either, which shows that hexose diphosphate and triose-P are not in equilibrium in this organism.