Prenatal sex determination from maternal peripheral blood using the polymerase chain reaction.

We have investigated the use of a nested polymerase chain reaction assay for the detection of a fetal-specific Y-chromosomal sequence (DYS14) from DNA extracted from unsorted maternal peripheral blood. Serial dilutions of male DNA into female cord blood DNA indicated that the assay could detect an equivalent of a single male cell in 300,000 female cells. The assay exhibited absolute specificity for male DNA with no amplification from a DNA panel obtained from 10 female cord blood samples.

Enhanced services and stipends for foster parents: effects on retention rates and outcomes for children.

Current national trends show that although the number of available foster homes is shrinking, the number of children and adolescents being cared for in the family foster care system is growing. This study demonstrates the significant benefits to both foster parents and the children in their care of providing enhanced services and stipends to foster parents.

A recombinant, chimeric enzyme with a novel mechanism of action leading to greater potency and selectivity than tissue-type plasminogen activator.

Background: Early intervention with thrombolytic agents has been shown unequivocally to reduce mortality after acute myocardial infarction. Presently used agents have disadvantages such as short half-life, immunogenicity, hypotension, and bleeding complications. Therefore, there is a need to develop improved thrombolytic drugs with novel mechanisms of action leading to improved properties.

Composite sonographic assessment of fetal health.

In this chapter the role of ultrasound scanning in the antenatal assessment of fetal growth, the biophysical profile score, and amniotic fluid volume is reviewed. Recent developments in these three areas are described with particular emphasis on assessment of fetal growth in the postmature and diabetic pregnancy. In addition, the false-negative test rate with the biophysical profile score and the role of ultrasound scanning in the assessment of amniotic fluid volume are outlined.

Uterine-peritoneal amniotic fluid leakage: an unusual complication of intrauterine shunting.

Bilateral pleuroamniotic shunting was performed at 33 weeks' gestation in a fetus with bilateral hydrothorax, hydrops, and gross polyhydramnios. The procedure was successful, but acute amniotic fluid leakage into the maternal peritoneal cavity occurred soon after. This produced marked maternal discomfort and transient oligohydramnios, with consequent fetal distress.

Late fetal death--has ultrasound a role to play in its prevention?

Late fetal death is the principle cause of perinatal mortality (PNM) in Ireland. Real-time ultrasound scanning has been suggested as a means for identifying the fetus at risk of abnormal pregnancy outcome. Using an ultrasound-based method of fetal assessment the author noted a significant reduction in late fetal death rates over a 4 year period (1985-1988: late fetal death rate: 3.

How accurate is antenatal sonographic identification of discordant birthweight in twins?

The association between discordant birth weight in twins and poor perinatal outcome is well recognised. Antenatal identification of discordant fetal growth in twins using ultrasound has been previously reported. In this study we have assessed the accuracy of interpair percentage estimated fetal weight difference determination (interpair EFW%) in identifying an interpair percentage birth weight difference (interpair BW%) of greater than or equal to 20% and greater than or equal to 25% in 85 twin pregnancies.

Severe limb abnormalities after chorion villus sampling at 56-66 days' gestation.

Among 289 pregnancies in which chorion villus sampling (CVS) was carried out at 56-66 days' gestation, 5 babies with severe limb abnormalities were subsequently identified. 4 had oromandibular-limb hypogenesis syndromes, and the other had a terminal transverse limb reduction defect. This high incidence raises the possibility that CVS was an aetiological factor for these developmental anomalies.

Interaction of a plasmin A-chain/t-PA B-chain hybrid enzyme with plasma inhibitors in vivo and in vitro.

A hybrid plasminogen activator consisting of the "A" chain of plasmin linked to the "B" chain of rt-PA was inhibited in vitro in human and guinea pig plasmas 4 to 5-fold more rapidly than its parent activator, two-chain t-PA. Using zymographic and autoradiographic techniques together with the use of immunodepleted plasma the major inhibitor was identified as alpha-2-antiplasmin. The pharmacokinetic profile of the hybrid in guinea pigs was determined by two different methods: disappearance of fibrinolytic activity and removal of radiolabelled hybrid from the circulation.

Serving the chronic mentally ill in state and community hospitals.

Acute psychiatric admissions to three state and three community hospitals from the same geographic areas were examined in terms of patient characteristics, services, and costs. Overall, patients in state hospitals were more likely than patients in community hospitals to be admitted involuntarily, to have bizarre or assaultive behaviors as a precipitating cause of admission, to have recent community mental health involvement, to be referred by family or friends, to be living in dependent care at admission, and to have police initiated admissions; differences on other variables such as prior psychiatric hospitalizations, or in-hospital behaviors were not significant. The length of stay was longer for state hospital patients who were also more likely to be discharged to an independent living situation.

Fetal biophysical profile scoring: selective use of the nonstress test.

The fetal biophysical profile score was modified by selective use of the nonstress test. In 2712 study patients (7851 tests) the incidence of nonstress test was reduced to 2.7% with no measurable effect or test accuracy.

Immunologic competence of hemodialysis patients following withdrawal of vitamin C supplement.

Twelve stable nondiabetic patients on chronic hemodialysis were studied for their immunobiologic competence while receiving vitamin C supplement and 1-2 months following its withdrawal. These subjects, on prescribed diets, were checked for their food intake at the beginning of the study and at 1-month intervals thereafter. Immunological parameters tested were: (1) plasma vitamin C levels; (2) number of mononuclear cells isolated from blood and their vitamin C contents; (3) lymphocyte blastogenic response to mitogens; (4) leukocyte metabolic functions in presence of endotoxin by nitroblue tetrazolium reduction test, and (5) delayed hypersensitivity to skin-testing antigens, e.

Identification of the small for gestational age fetus with the use of gestational age-independent indices of fetal growth.

This study reviews the roles of sonographic assessment of the rate of growth of the fetal abdominal circumference, the femur length/abdominal circumference ratio, and qualitative determination of amniotic fluid volume as gestational age-independent indices for identification of the small for gestational age fetus. The sensitivity and specificity for single and combinations of test results were evaluated in 50 appropriate for gestational age and 40 small for gestational age fetuses. Positive and negative predictive values were derived for the general population.