Purification and Biological Activities of Enzymatically Degraded Sargassum fusiforme Polysaccharides.

Enzymatic hydrolysate of the crude polysaccharide (SFP) extracted from Sargassum fusiforme was purified by column DEAE-52 and Sephadex G-100 to yield four components, namely, ESFP1, ESFP2, ESFP3 and ESFP4. These components were characterized by chemical composition assay, GC/MS, HPGPC, UV and FT-IR techniques. The in vitro antioxidant activities of the four purified fractions were investigated by measuring their radical scavenging activity and reducing power.

Carboxymethylation of polysaccharides: Synthesis and bioactivities.

Polysaccharides are a structurally diverse class of biomolecules with a wide variety of bioactivities. Natural polysaccharides isolated from plants and fungi are used as raw materials in food and pharmaceutical industries due to their therapeutic properties, non-toxicity, and negligible side effects, but many natural polysaccharides possess low bioactivities when compared to synthetic medicines due to their structure and physicochemical properties. Literature studies revealed that carboxymethylation of polysaccharides enhances the bioactivities and water solubility of native polysaccharides significantly, and provide structural diversity and even the addition of new bioactivities.

Novel menadione hybrids: Synthesis, anticancer activity, and cell-based studies.

A series of novel menadione-based triazole hybrids were designed and synthesized by employing copper-catalyzed azide-alkyne cycloaddition (CuAAC). All the synthesized hybrids were characterized by their spectral data ( H NMR, C NMR, IR, and HRMS). The synthesized compounds were evaluated for their anticancer activity against five selected cancer cell lines including lung (A549), prostate (DU-145), cervical (Hela), breast (MCF-7), and mouse melanoma (B-16) using MTT assay.

Synthesis and anticancer activity of some novel 5,6-fused hybrids of juglone based 1,4-naphthoquinones.

Six novel 5,6-fused hybrids such as dihydrobenzofuran-quinone (4a and 4b), benzofuran-quinone (5a and 5b) and chromene-quinone (6a and 6b) of juglone based 1,4-naphthoquinones were synthesized by employing a three step protocol with the cyclisation of o-allyl phenol as the key step. The anticancer activity of the newly synthesized compounds was evaluated in vitro against seven human cancer cell lines including cervix (ME-180 and HeLa), breast (MCF-7, MDA-MB-453 and MDA-MB-231), prostate (PC-3) and colon (HT-29) by using MTT assay. The screening results showed that majority of the synthesized compounds exhibited significant anticancer activity.