An in vivo study of the chlorhexidine release profile of the PerioChip in the gingival crevicular fluid, plasma and urine.

The release profile of chlorhexidine from the PerioChip (Chip), a biodegradable local delivery system that contains 2.5 mg of chlorhexidine gluconate (CHX) in a cross-linked hydrolyzed gelatin matrix, into the gingival crevice, was evaluated in an in vivo, open label, single-center, 10-day pharmacokinetic study conducted on 19 volunteers with chronic adult periodontitis. Each volunteer had a single chip inserted into each of 4 selected pockets, with probing pocket depths of between 5-8 mm, at time 0.

The effect of cyclosporin A (CsA) treatment on gingival tissue of patients with Behçet's disease.

Gingival biopsies were obtained at various intervals for a period of two years from 12 patients suffering from Behçet's disease who were under a clinical trial of cyclosporin A (CsA) treatment. The levels of nondialyzable hydroxyproline (Hypro) were determined in the medium of the cultured tissues. Histologic examinations were also performed every three months.

Evaluation of conventional therapy versus cyclosporine A in Behçet's syndrome.

When ocular structures are affected in Behçet's syndrome, a rapid loss of vision has been the rule regardless of the type of treatment--corticosteroids, Leukeran, Imuran, colchicine. The objectives of the present study were to compare, in a masked manner, conventional treatment (corticosteroids/Leukeran) to treatment with CsA. Forty patients suffering from Behçet's syndrome with active ocular inflammatory reactions were included in the study.

Treatment of endogenous uveitis with cyclosporine A.

Thirty patients suffering from severe sight-threatening bilateral chronic endogenous uveitis were treated with initial dosages of 10 mg/kg/d of CsA. After 1 month of therapy, all patients but one demonstrated a rapid decrease of the intraocular inflammatory processes along with an arrest of the deterioration of vision. After 1 year (or more) of treatment, most patients still show the same visual acuity achieved after 1 month.

Interaction of lecithin:cholesterol acyltransferase and cholesteryl ester transfer protein in the transport of cholesteryl ester into sphingomyelin liposomes.

When isolated lecithin:cholesterol acyltransferase was incubated with cholesterol-lecithin liposomes in the presence of apolipoprotein A-1, cholesteryl ester accumulated until a maximal ester/lecithin weight ratio of 0.03 was reached. This was independent of the amount of enzyme present or the proportion of cholesterol relative to lecithin.

Isolation and characterization of a human serum cholesteryl ester transfer protein.

Human plasma has been shown to contain an apolipoprotein that mediates the transport of cholesteryl ester from high density lipoprotein (HDL) to very low density lipoprotein (VLDL) or low density lipoprotein (LDL). This activity, confined to the density greater than 1.063 g/ml interval, has been isolated from HDL and appears as a single migrating species by anionic or sodium dodecyl sulfate/polyacrylamide gel electrophoresis.

Very low density lipoprotein. Metabolism of phospholipids, cholesterol, and apolipoprotein C in the isolated perfused rat heart.

The fate of rat plasma very low density lipoprotein (VLDL) constituents was determined in the isolated perfused rat heart. VLDL was labeled with [(14)C]palmitate, (32)P-phospholipids, [(3)H] cholesterol, and (125)I-apolipoprotein C (apoC). Perfusions were performed with an albumin-containing buffer and without plasma.

Lipoprotein lipase of cultured mesenchymal rat heart cells. I. Synthesis, secretion and releasability by heparin.

Cell suspensions prepared from rat hearts were separated by replating into F1, F2 and M cultures, and cultured for 3--11 days. Lipoprotein lipase activity was highest in the F1 cultures which consisted mainly of non-beating, mesenchymal cells. The enzyme activity was released into the medium only after addition of heparin.

A genetic study of Behçet disease in Israel.

The frequency of HLA-B5 in 24 Israeli Behçet disease (BD) patients from various subpopulations was significantly greater than in 615 control individuals (P less than 0.003). The relative risk for a B5 carrier to develop BD was calculated to be 5.

Rat heart in culture as a tool to elucidate the cellular origin of lipoprotein lipase.

Lipoprotein lipase was determined in 5-day old cell cultures derived from hearts of newborn rats. With the help of the preplating method the cells were subdivided into cultures containing predominantly cardiac myocytes and into those composed mainly of mesenchymal cells. Lipoprotein lipase activity, associated with the mesenchymal cells was ten times higher than the activity found in the cultures containing mainly the myogenic cells.

Treatment of acute hepatic encephalopathy with L-dopa.

A clinical report of a 30-year-old woman who developed acute hepatic failure in the fifth month of pregnancy is presented. L-dopa administration resulted in a marked improvement in both level of consciousness and electroencephalogram. The literature dealing with L-dopa therapy in hepatic encephalopathy is reviewed.

Pre- and post-natal development of lipoprotein lipase and hepatic triglyceride hydrolase activity in rat tissues.

The ontogenic development of lipoprotein lipase and liver triglyceride hydrolase was studied in the rat. The enzyme activity measured in extrahepatic tissues fulfilled the criteria of lipoprotein lipase from the onset of measurable activity, i.e.

Ultrastructural localization of concanavalin A in the perfused rat heart.

Staining of the surface coat of heart capillaries and aorta was achieved by perfusing concanavalin A and phytohemagglutinin coupled to horseradish peroxidase through the rat heart. The binding of these lectins provides evidence for the presence of glycoproteins containing mannose, glucose, and N-acetylgalactosamine residues in the endothelial surface coat of rat aorta and heart capillaries. The binding of concanavalin A to capillary endothelium was further followed with radioautography of 125I-labeled concanavalin A in the in vitro perfused rat heart.

Interaction of concanavalin A with membrane-bound and solubilized lipoprotein lipase of rat heart.

Concanavalin A was used to study the configuration of lipoprotein lipase at the surface of capillary endothelium. Incubation of heart homogenates with increasing concentrations of concanavalin A for 5-60 min resulted in inhibition of up to 50% of enzyme activity. The inhibition was related to the concentration of lectin and the time of incubation and was fully reversible by postincubation with alpha-methyl-D-mannoside.

Quinidine hypersensitivity and liver involvement. A survey of 32 patients.

Thirty-two of 487 patients who received quinidine between the years of 1970 and 1974 in the departments of medicine at Hadassah University Hospital developed hypersensitivity reactions. Ten of the 32 patients had clinical and biochemical manifestations of liver invlovement. In 4 patients liver biopsy showed granulomatous hepatitis.

Cushing syndrome with cyclical edema and periodic secretion of corticosteroids.

A patient had a malignant carcinoid tumor of the lung. Cyclical edema was closely associated with periodic secretion of corticosteroids. Surgical excision of the tumor reversed the clinical and biochemical abnormalities.

Non-invasive quantitation of corneal copper in hepatolenticular degeneration (Wilson's disease).

The corneal copper content was measured by X-ray excitation spectrometry in two controls and in seven patients with Wilson's disease. Patients who were treated irregularly or not treated at all showed a high corneal copper content. Those who were adequately treated had low levels, comparable to the controls.

Interference with the transport of heparin-releasable lipoprotein lipase in the perfused rat heart by colchicine and vinblastine.

The effect of pretreatment with colchicine or vinblastine on the lipoprotein lipase activity of rat heart was studied. Administration of colchicine or vinblastine 4 h prior to perfusion of the heart caused a very marked reduction in lipoprotein lipase activity released into the perfusate within 1 min of heparin perfusion. At the same time an increase in residual heart lipase occurred so that total lipoprotein lipase content of the heart (heparin releasable plus residual) did not change.

Behçet's disease. Report of 41 cases and a review of the literature.

Several important conclusions may be derived on the basis of our experience with Behcet's disease (B.D.) and a review of the literature, namely: 1.