RNA-Sequencing of Heterorhabditis nematodes to identify factors involved in symbiosis with Photorhabdus bacteria.

Background: Nematodes are a major group of soil inhabiting organisms. Heterorhabditis nematodes are insect-pathogenic nematodes and live in a close symbiotic association with Photorhabdus bacteria. Heterorhabditis-Photorhabdus pair offers a powerful and genetically tractable model to study animal-microbe symbiosis.

Nematode genome announcement: The draft genome sequence of entomopathogenic nematode .

is one of the most widely used entomopathogenic nematodes for the biological control of agricultural insect pests worldwide. The draft genome of was sequenced using three genomic libraries of 300 bp, 600 bp and 5 kb sizes by Illumina HiSeq platform. The size of the draft genome assembly was 91.

sp. nov., subsp. subsp. nov. and subsp. subsp. nov. isolated from entomopathogenic nematodes.

Two Gram-negative, rod-shaped bacteria, H1 and H3, isolated from the digestive tract of entomopathogenic nematodes were biochemically and molecularly characterized to determine their taxonomic positions. The 16S rRNA gene sequences of these strains indicate that they belong to the Gammaproteobacteria, to the family , and to the genus. Deeper analyses using whole genome-based phylogenetic reconstructions show that strains H1 and H3 are closely related to DSM 15138, to DSM 22397, and to PB45.

Rapid and sensitive detection of potato cyst nematode by loop-mediated isothermal amplification assay.

Unlabelled: Cyst nematodes of the species and are devastating parasites of the potato crop. Early detection of cyst nematodes in the field is critical for adopting an appropriate management strategy. A specific and sensitive loop-mediated isothermal amplification (LAMP) assay using four oligonucleotide primers has been developed to amplify the internal transcribed spacer region (ITS) of ribosomal DNA of potato cyst nematode The PCN-LAMP reaction could be completed within 75 min at 68 °C followed by termination at 85 °C for 7 min.

An improved draft genome assembly of Meloidogyne graminicola IARI strain using long-read sequencing.

The rice root-knot nematode Meloidogyne graminicola is a major biotic stress for the rice crop under upland, rain-fed lowland and irrigated cultivation conditions. Here, we present an improved draft genome assembly of M. graminicola IARI strain using the long-read sequencing approach (PacBio Sequel platform).