Discovery of two bifunctional/multifunctional cellulases by functional metagenomics.

Cellulases are widely used in industry, and the usage in bioconversion of biofuels makes cellulases more valuable. In this study, two tandem genes that encoded cellulases ZF994-1 and ZF994-2, respectively, were identified on a cosmid from a soil metagenomic library. Phylogenetic analysis indicated that ZF994-1 and ZF994-2 belonged to glycoside hydrolase family 12 (GH12), and GH3, respectively.

Genome-Wide Analysis of the Gene Family and Expression Analysis during Anther Development in .

genes constitute a large family of transcription factors that play important roles in plant growth and development. However, our understanding of genes involved in anther development and male sterility in is still limited. In this study, 63 genes were identified from the genome of the male sterility ecotype Sichuan (_SC) unevenly distributed among eight chromosomes.

Early lateral spread response loss during microvascular decompression for hemifacial spasm: its preoperative predictive factors and impact on surgical outcomes.

When early lateral spread response (LSR) loss before decompression in HFS surgery happens, the value of intraoperative monitoring of LSR for locating neurovascular conflicts and confirming adequate decompression was considered to be reduced. This study aimed to identify preoperative parameters predicting early LSR loss and figure out the impact of early LSR loss on prognosis. Hemifacial spasm (HFS) patients who received microvascular decompression (MVD) under intraoperative electrophysiological monitoring during the period of March 2013-January 2021 were reviewed retrospectively.

Improved Optical Path Structure for Symmetric Demodulation Method in EFPI Fiber Optic Acoustic Sensors Using Wavelength Division Multiplexing.

This paper presents a novel improvement in the optical path structure of a three-wavelength symmetric demodulation method applied to extrinsic Fabry-Perot interferometer (EFPI) fiber optic acoustic sensors. The traditional approach of using couplers to construct the phase difference in the symmetric demodulation method is replaced with a new approach that combines the symmetric demodulation algorithm with wavelength division multiplexing (WDM) technology. This improvement addresses the issue of a suboptimal coupler split ratio and phase difference, which can affect the accuracy and performance of the symmetric demodulation method.

Internal validation of an improved system for forensic application: a 41-plex Y-STR panel.

Unlabelled: Y-chromosome short tandem repeats (Y-STRs) have a unique role in forensic investigation. However, low-medium mutating Y-STRs cannot meet the requirements for male lineage differentiation in inbred populations, whereas rapidly mutating (RM) high-resolution Y-STRs might cause unexpected exclusion of paternal lineages. Thus, combining Y-STRs with low and high mutation rates helps to distinguish male individuals and lineages in family screening and analysis of genetic relationships.

Orthotic intervention options to non-surgically manage adult and pediatric trigger finger: A systematic review.

Background: The design and efficacy of orthotic intervention to non-surgically manage adult and pediatric trigger finger vary widely.

Purpose: To identify types of orthoses (including relative motion), effectiveness, and outcome measurements used to non-surgically manage adult and pediatric trigger finger.

Study Design: Systematic review.

Robotic-assisted double lingual mucosal graft ureteroplasty for multifocal ureteral strictures: Case report and technical description.

Background: Ureteroplasty with a single onlay graft for proximal ureter stricture has been widely used in the clinic. However, robotic ureteroplasty with a double lingual mucosal graft (RU-DLMG) has not been reported.

Methods: The intraoperative measured ureteral stricture lengths of patient 1 were 1.

Integrated Multiomics and Synergistic Functional Network Revealed the Mechanism in the Tolerance of Different Ecotypes of Bge. to Doxycycline Pollution.

Tetracycline pollution in soil irreversibly damages the biosafety of plants by inhibiting the mitochondrial function. Some traditional Chinese medicine (TCM) plants, such as Bunge, have a strong tolerance to mitochondrial damage. We comprehensively compared the doxycycline (DOX) tolerances of two ecotypes of in the Sichuan and Shandong provinces and found that the Sichuan ecotype had a lower yield reduction, more stable accumulation of medicinal ingredients, higher mitochondrial integrity, and a more robust antioxidant system.

Genomic evidence reveals high genetic diversity in a narrowly distributed species and natural hybridization risk with a widespread species in the genus Geodorum.

Background: Understanding genetic diversity is a core issue in conservation genetics. However, previous genetic diversity evaluations of narrowly distributed species have rarely used closely related widespread species as a reference. Furthermore, identifying natural hybridization signals between narrowly and widely distributed sympatric species is of great importance for the development of species conservation programs.

Strong ubiquitous micro-promoters for recombinant adeno-associated viral vectors.

Significant progress has been made in developing recombinant adeno-associated virus (rAAV) for clinical gene therapy. While rAAV is a versatile gene delivery platform, its packaging limit of 4.7 kb limits the diseases it can target.

Semi-Solid Supramolecular Ionic Network Electrolytes Formed by Zwitterionic Liquids and Polyoxometalate Nanoclusters for High Proton Conduction.

Flexible electrolytes with solid self-supporting properties are highly desired in the fields of energy and electronics. However, traditional flexible electrolytes prepared by doping ionic liquids or salt solutions into a polymer matrix pose a risk of liquid component leakage during device operation. In this work, the development of supramolecular ionic network electrolytes using polyoxometalate nanoclusters as supramolecular crosslinkers to solidify bola-type zwitterionic liquids is reported.

Research on production capacity planning method of open-pit coal mine.

Reasonable production capacity is related to the economic benefits of an open-pit coal mine. This study analyzes the relationship between the working face length, the annual advancing speed and the production capacity. It constructs a production capacity function relationship model.

[Relationship between cadmium exposure and pulmonary function level and chronic obstructive pulmonary disease].

To analyze the levels and distribution characteristics of blood cadmium and urinary cadmium in American adults, to analyze the relationship between blood cadmium and urinary cadmium and pulmonary function dose response, and to explore the effect of this index on the risk of chronic obstructive pulmonary disease. In March 2022, 3785 patients from 2007 to 2012 in NHANES database were selected as the subjects. Collect demography data such as gender and age, and test data such as lung function, blood cadmium concentration and Urine cadimium concentration.

Addressing barriers to increased adoption of DPYD genotyping at a large multisite cancer center.

Purpose: To describe the implementation of an in-house genotyping program to detect genetic variants linked to impaired dihydropyrimidine dehydrogenase (DPD) metabolism at a large multisite cancer center, including barriers to implementation and mechanisms to overcome barriers to facilitate test adoption.

Summary: Fluoropyrimidines, including fluorouracil and capecitabine, are commonly used chemotherapy agents in the treatment of solid tumors, such as gastrointestinal cancers. DPD is encoded by the DPYD gene, and individuals classified as DPYD intermediate and poor metabolizers due to certain genetic variations in DPYD can experience reduced fluoropyrimidine clearance and an increased risk of fluoropyrimidine-related adverse events.

Coordination-Controlled Catalytic Activity of Cobalt Oxides for Ozone Decomposition.

Nowadays, it is still elusive and challenging to discover the active sites of cobalt (Co) cations in different coordination structures, though Co-based oxides show their great potency in catalytic ozone elimination for air cleaning. Herein, different Co-based oxides are controllably synthesized including hexagonal wurtzite CoO-W with Co in tetrahedral coordination (Co) and CoAl spinel with dominant Co, cubic rock salt CoO-R with Co in octahedral coordination (Co), MgCo spinel with dominant Co in octahedral coordination (Co), and CoO with mixed Co and Co. The valences are proved by X-ray photoelectron spectroscopy, and the coordinations are verified by X-ray absorption fine structure analysis.

Tunable Organic Active Neural Probe Enabling Near-Sensor Signal Processing.

Neural recording systems have significantly progressed to provide an advanced understanding and treatment for neurological diseases. Flexible transistor-based active neural probes exhibit great potential in electrophysiology applications due to their intrinsic amplification capability and tissue-compliant nature. However, most current active neural probes exhibit bulky back-end connectivity since the output is current, and the development of an integrated circuit for voltage output is crucial for near-sensor signal processing at the abiotic/biotic interface.

LENS: Landscape of Effective Neoantigens Software.

Motivation: Elimination of cancer cells by T cells is a critical mechanism of anti-tumor immunity and cancer immunotherapy response. T cells recognize cancer cells by engagement of T cell receptors with peptide epitopes presented by major histocompatibility complex molecules on the cancer cell surface. Peptide epitopes can be derived from antigen proteins coded for by multiple genomic sources.

Effect of sarcopenia, osteoporosis, and osteosarcopenia on spine fracture in American adults with prediabetes.

Objective: This study aimed to investigate the effect of sarcopenia, osteoporosis, and osteosarcopenia on spine fracture in patients with prediabetes.

Methods: We collected and analyzed the data from the U.S.