Reactive oxygen FIB spin milling enables correlative workflow for 3D super-resolution light microscopy and serial FIB/SEM of cultured cells.

Correlative light and electron microscopy (CLEM) is a powerful tool for defining the ultrastructural context of molecularly-labeled biological specimens, particularly when superresolution fluorescence microscopy (SRM) is used for CLEM. Current CLEM, however, is limited by the stark differences in sample preparation requirements between the two modalities. For CLEM using SRM, the small region of interest (ROI) of either or both modalities also leads to low success rate and imaging throughput.

Ion mobility studies of electronically excited States of atomic transition metal cations: development of an ion mobility source for guided ion beam experiments.

The design of an ion mobility source developed to couple to a guided ion beam tandem mass spectrometer is presented. In these exploratory studies, metal ions are created continuously by electron ionization of the volatile hexacarbonyls of the three group 6 transition metals. These ions are focused into a linear hexapole ion trap, which collects the ions and then creates high intensity pulses of ions, avoiding excessive ion losses resulting from the low duty cycle of pulsed operation.

On the Structural Dichotomy of Cationic, Anionic, and Neutral FeS(2).

Structural and thermochemical aspects of the FeS(2)(+) cation are examined by different mass spectrometric methods and ab initio calculations using density functional theory. Accurate threshold measurements provide thermochemical data for FeS(+), FeS(2)(+), and FeCS(+), i.e.