[Laparoscopy of the upper urinary tract].

The upper urinary tract is a wide field for using of laparoscopy. The aim of this work was to analyze retrospectively our experience in the development of laparoscopic techniques of surgical treatment of upper urinary tract pathology. 137 patients with pathology of the upper urinary tract were operated laparoscopically in our department during three years (July 2010 - July 2013).

[Pharyngolaryngeal reflux as one of the causes of chronic excretory otitis media].

The objective of the present work was to study the relationship between chronic secretory otitis media (CSOM) and pharyngolaryngeal reflux (PLR). A total of 43 patients aged between 3 to 19 years presenting with CSOM were available for the examination. PLR was confirmed in 36 (83.

[Aspartate aminotransferase metabolism in muscles during physical exercise].

It is established that the concentration of cytosolic aspartate aminotransferase decreases the first day after intensive physical exercises due to the intensification of the degradation processes, the decrease in the synthesis rate and the enzyme entry into the blood vessel. The enzyme content is normalized by the 24th hour of rest and then the supercompensatory phase takes place: the enzyme biosynthesis increases and its degradation decreases to a greater extent.

[Androgen regulation of the level of tissue-specific proteins in skeletal muscles during physical exercise].

Administration of testosterone after exercises led to suppression of the hormone production during the anabolic phase within 72-120 hrs of muscles adaptation to physical loading. Content of the androgen receptors was also altered. The supercompensatory phase in content of proteins (myoglobin and aspartate aminotransferase as index) was absent due to an impairing effect of testosterone on muscle adaptation.

[Radioimmunologic analysis of steroids. Scientific and practical aspects].

Many-year experience of using authors' own systems for steroid radioimmunoassay (RIA) is summarized. Synthesis of natural and anabolic steroid derivatives and their conjugation with bovine serum albumin are considered. Schemes of rabbits immunization with steroid-protein conjugates, and the antisera characteristics are described.

[Tropomyosin metabolism in muscles and its blood levels during physical exertion].

Physical exercise induces phasic changes in the tropomyosin content and metabolism in muscles and its concentration in blood. The intensive catabolic processes (decrease of 14C-leicin inclusion and time of half-life) of muscle tropomyosin and its appearance in blood were shown 2-24 hours after the exercise. Intensive anabolic processes of muscle tropomyosin were found at the late period of rest (72-144 h).

[Effect of physical exercise on myoglobin and tropomyosin levels in skeletal muscles and myoglobin level in rat blood].

Single intensive physical exercise caused phase alterations in content of myoglobin and tropomyosin in rat skeletal muscles. Within the first 2-4 hrs of rest concentration of myoglobin and tropomyosin was decreased in muscles by 40-50% (catabolic, urgent step of adaptation). Within the later period of rest (72-120 hrs) content of myoglobin and tropomyosin was increased by 25-30% (anabolic, later step of adaptation) as compared with the control level.

[The steroid hormone levels and receptors of steroid hormone receptors in rat muscles during physical exertion].

It is shown that the testosterone content in skeletal muscles of female albino rats is 2-fold decreased, while the estradiol content-1.5-fold increased and progesteron content showed no changes after systematic physical exercises. The pharmacokinetic investigations showed that androgen half-life in the organism decreased from 8 to 5 h under physical exercises.

[Steroid levels and androgen receptors in skeletal muscles during adaptation to physical effort].

Influence of physical exercises on the content of androstendione, estradiol and testosterone was studied in blood and skeletal muscles. The content of steroids was increased immediately after physical exercises and diminished down to the initial level within 48-72 hrs. Androgen receptors were increased from 0.

[Radioimmunologic assay of steroids. Comparison of 3H- and 125I-ligands].

Characteristic properties of radioimmunoassay (RIA) of steroids with 3H- and 124I-ligands as a label were studied using two anabolic steroids methandrostenolone and 19-nortestosterone. Antisera towards 124I-steroids were used at higher dilutions as their specific activity was distinctly more than in the antisera to 3H-substances. However, 125I-steroid must be in a stereochemical correspondence with the antiserum otherwise antibodies do not bind the label.

[Content and utilization of testosterone in the cardiac and skeletal muscles of male rats during physical exercise].

They influenced differently the content, transport and utilization of testosterone (T) in the body. The strength training resulted in a 2.5-3-fold increase of the T-content and binding capacity of blood proteins as compared with untrained animals, and a 1.

[Radioimmunologic determination of androstenedione and the effect of physical training on its levels in human blood and saliva].

A radioimmunoassay (RIA) of androstenedione in biological fluids was developed. The sensitivity of the method was 30 pg/ml. The efficiency of the standard detection in blood and saliva was 98.

[Effect of the conditions of isolation of free and bound ligands on the accuracy of the radioimmunoassay of steroids].

Testosterone radioimmunoassay (RIA) was used to analyse the factors which influenced the precision and accuracy of steroid RIA. Selection of optimal parameters (concentrations of activated charcoal and of the radioactive steroid) was necessary in order to reduce the influence of non-specific binding and error of fractionation on the RIA results. The scheme for estimation of the optimal values of these parameters is described.

[Testosterone concentrations in human biological fluids following various routes of administration].

The antiserum against testosterone (T) was obtained and its titer-dependent specificity was studied during the rabbit immunization. The radioimmunoassay for T determination in the human saliva, blood serum and urine was developed. The sensitivity is 6 pg, the variation coefficient within the experiment is 5.

[Radioimmune determination of steroids--comparison of 2 variants of the methods].

Various conditions of radioimmunoassay (RIA) technique for estimation of 19-nortestosterone and methandrostenolone were studied. Dilution of antiserum, required for 50% binding of the labelled steroids, depended primarily on the RIA procedure used. When 19-nortestosterone was estimated by means of these two RIA procedures, the final dilution of antiserum was equal to 1:24,000 and 1:475,000, respectively.