The Centrosomes of Haploid and Diploid Cells Have an Equal Number of Centrioles in the Parasitoid Wasp .

Background: The centrosome is the main center of the organization of microtubules (MT) in the cell, the origin for the formation of flagella and cilia, as well as the site of many regulatory intracellular processes. In diploid cells, the centrosome includes two centrioles connected to some additional structures and surrounded by pericentriolar material.

Methods: The ultrastructure of the cells was studied using transmission electron microscopy on serial ultrathin sections.

Regeneration of Rabbit Auricular Cartilage After the Intravenous Stem Cell Injection.

Background: The restoration of auricular cartilage is a major problem of otolaryngology. The low regenerative capacity of cartilage requires alternative approaches such as cell and tissue engineering. Stem cells are one of the ways to repair auricular cartilage damages.

[Mass spectrometry virulence marker Vibrio cholerae.].

Identification mass-spectrometry of marker proteins of toxicity of V. cholerae for development the express of diagnostics of the causative agent of cholera on the basis of the computer analysis in the MALDI-ToF format of electronic profiles became the purpose of our research. Subjected to the computer analysis mass and spectrometer electronic passports 140 of strains of V.

[The mass-spectrometric analysis of MALDI-TOF in identification and typing of strains of comma bacillus].

The data base “Protein profiles of mass-specters of representatives of species of Vibrio cholerae for program MALDI Biotyper” was used to implement typing of strains of comma bacillus isolated at the territory of the Russian Federation in 2010-2012. Also, analysis of degree of similarity and differences among constant ribosomal proteins was implemented. According the results of MALDI-TOF mass-spectrometry strains of V.

[The time-of-flight mass spectrometer intra-specific diagnostic of strains of Escherichia isolated from patient].

The time-of-flight mass spectrometer analysis of freshly separated human colibacillus populations for identification of markers of proteom specific for hemolytic and non-hemolytic strains, Escherichia-associant of opportunistic enterobacteria. The material consisted of 20 strains of mono-population of hemolytic Escherichia; 100 strains of mono-population of non-hemolytic Escherichia; 15 non-hemolytic strains isolated from association with opportunistic enterobacteria. The protein profiling was implemented on mass spectrometer MALDI-TOF MS Autoflex “Bruker Daltonik”.

[POSSIBILITIES OF APPLICATION OF MALDI-TOF MASS-SPECTROMETRY FOR STUDY OF CARBOHYDRATE-SPECIFIC RECEPTORS FOR DIAGNOSTIC BACTERIOPHAGE EL TOR].

Aim: Study mechanisms of interaction of diagnostic bacteriophage El Tor with sensitive strain Vibrio cholerae El Tor 18507 using direct protein profiling, identification of constant and variable proteins, taking part in interaction of the phage and cell, as well as carbohydrate-specific phage receptors.

Materials And Methods: . A commercial preparation of cholera diagnostic bacteriophage El Tor, strain V.

[PROTEOMIC MASS-SPECTROMETRIC ANALYSIS OF FRESHLY ISOLATED ESCHERICHIA COLI STRAINS].

Aim: Time-of-flight mass-spectrometric analysis of colon bacilli populations freshly isolated from humans for identification of proteome markers, that are characteristic for hemolytic and non-hemolytic strains, E.coli--associants of opportunistic bacteria.

Materials And Methods: 20 strains of monopopulation of hemolytic E.

[The differentiation of specimiens of Vibrio parahaemolyticua and Vobrio alginolyticus].

Vibrio parahaemolyticua and Vobrio alginolyticus are phylogenetically closely-related species. They have common ecological niches, same cultural features and similar biochemical characteristics. The phenotype variability and taxonomy similarity of strains of these species impedes differentiation of Vibrio parahaemolyticua and Vobrio alginolyticus according biochemical characteristics.

[The application of mass spectrometry technique MALDI-TOF for inter-specific differentiation of closely-related vibrio].

The study was targeted to apply mass spectrometry analysis for inter-specific differentiation of collection strains of representatives of genus vibrio and identification of comma bacilli extracted from samples of boat ballast waters. The samples consisted of 207 museum strains of cultures and 347 microorganisms from samples of boat ballast waters. The identification of microorganisms was implemented using MALDI-TOF mass spectrometers.

[Cytopathological effect of Bacillus thuringiensis israelensis endotoxins on the intestines of Aedes aegypti mosquito larvae].

The dynamics of pathological changes in the intestine of Aedes aegypti larvae under the influence of toxins Cry11A and Cry4B produced by Bacillus thuringiensis israelensis was studied by means of electron microscope. Most significant ultrastructure changes in the intestine of the second instar larvae were observed in the midgut. The cytoplasm of cells disintegrated, and elongated lacunae appeared.

[Parasitism--the existence of organisms as a component of parasitic systems].

Attempt to give a definition of the parasitism phenomenon from the symbiotological point of view is proposed. Parasites are the organisms, existence of which is possible only in the parasitic systems of any degree of complexity. Outside the parasitic system the parasite is not capable to realize its main vital function including feeding, dispersion of reproduction.

[The means for the morphofunctional transformations of hematophagous insects].

Morphofunctional transformations of hematophagous insects, connected with forming the type of parasitism is performed by different forms of adaptation morphosis. Most morphological changes occur within the framework of idioadaptations (allomorphoses); which is especially characteristic of all free-living hematophagous groups. The transition of hematophagues to ectoparasitism, especially permanent, is accompanied, besides idioadaptations, by cenogenetic shifts (pupigenous dipterans, hemipterans), as well as by processes of degradation of organs, with a generally hypomorphous development of a group (lice).

[Ultrastructural characteristics of the simple eyes of the louse Pediculus humanus corporis].

The simple eye of the human louse consists of two apparatuses: dioptric and light sensitive. The dioptric apparatus contains only a biconvex lens, which represents local thickening of the cuticle. The eye lacks the crystal cone (Semper cells) and special pigment cells.

[Micromorphology of the malpighian tubules in the louse Pediculus humanus corporis (Anoplura)].

The ultrastructure of the Malpighian tubes in human louse Pediculus humanus corporis has been studied. The cells of the Malpighian tubules have the uniform structure: the apical surface is covered with microvilli, the basal plasmatic membrana forms relatively small invaginations. The microvilli are most developed in cells of the proximal department of the Malpighian tubules.

[Ultrafine structure of the malpighian tubules of hematophagous Diptera].

The ultrastructure of Malpighian tubes of 5 species of bloodsucking Diptera was studied: Culicoides pulicaris, Tabanus bromius, Hybomitra schineri, Haematopota pluvialis and Stomoxys calcitrans. The Malpighian tubes of the above species include the cells of two types. The most abundant cells of the 1st type contain many spherical inclusions which represent deposits of mineral compounds.

[Electron microscopic study of the malpighian tubules in fleas (Siphonaptera)].

A study was carried out of ultrastructure of malpighian tubules of 4 species of fleas as follows: Xenopsylla cheopis, Ceratophyllus fasciatus, Leptopsylla segnis, Ctenophthalmus orientalis. All cells of malpighian tubules are of polar structure: their apical department is represented with microvilli while basal one--with a system of membrane invaginations forming the basal labyrinth. The most developed system of basal invaginations is observed in distal cells of the malpighian tubules.

[Comparative characteristics of the digestive enzymes of blood-sucking insects. II. Invertase and lipase].

The activity of invertase and lipase in the midguts of 11 hematophagous insect species have been determined. The highest invertase activity at pH 7-10. Insects feeding on carbohydrate meals as well as with blood have the greatest invertase activity, the obligatory blood feeders have the least one.

[Evaluation of the interrelations among auditory potential parameters by means of the technic of correlation-regression analysis].

The characteristics (amplitude, latency) of two types of evoked potentials, action potential of the 8th nerve recorded at the cochlea round window and sonomotor evoked potential recorded from the postauricular region and the back of the guinea pigs were studied in response to sound stimulation. The degree and type of interrelation between the parameters of the auditory evoked potentials were established employing the method of correlation-regression analysis. A close linear dependence was established for amplitude values ( r = 0.

[Functional morphology of the midgut in satiated Pediculus humanus corporis (Anoplura) fleas].

It has been shown that during feeding the midgut epithelium of the human louse Pediculus humanus corporis consists of three types of cells: secretory, digestive, and reserve (regenerative) ones. According to the ultrastructural organization the secretory cells are subdivided into two regions--apical and basal. In the apical region of the secretory cells no microvilli are observed.

[Ultrastructure of the antennal sensillae of the bug, Rhodnius prolixus (Hemiptera, Reduviidae)].

On the antenna of the bug R. prolixus we found numerous receptors which can be classified into three categories: mechanoreceptors (sensilla chaetica), olfactory (sensillae trichodea, basiconica and caeloconica) and gustatory (sensilla trichodea) receptors. The number, size and distribution on the antenna of each type have been recorded.