Publications by authors named "Ch Sasikala"

Article Synopsis
  • The study aimed to explore how hydrogen peroxide (H2O2) affects the survival of Bacillus subtilis spores, focusing on wild-type and a sporulene-deficient mutant.
  • Treatment with H2O2 reduced the viability of the sporulene-deficient mutant by 80%, revealing significant damage manifested in altered spore morphology and compromised membrane integrity.
  • The findings indicated that sporulenes provide crucial protection against H2O2 toxicity, with the wild-type strain showing enhanced growth and sporulene production as a defensive response compared to the mutant strain.
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Sporulene, a pentacyclic triterpenoid, was discovered in Bacillus subtilis and is associated with bacterial endospores. However, the study was not further extended, leaving a trail of questions. One such question is what diversity of sporulenes exists among spore-forming members? Considering the sporulene biosynthesis pathway as a fundamental tool to survey the distribution of this molecule, a genome mining study was conducted.

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The development of an efficient route for the synthesis of Canagliflozin is reported. The anhydroketopyranose intermediate was isolated as a novel intermediate, which was used to prepare Canagliflozin API in high purity.

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Four strains of Planctomycetes, (JC636, JC649, JC650, JC657) which are all salt and alkali tolerant, pink coloured, with spherical to oval shaped, Gram-stain-negative, non-motile cells were isolated from different regions of Chilika lagoon, India. All strains have obligate requirement for N-acetylglucosamine (NAG) and share highest 16S rRNA gene sequence identity with members of the genus Tautonia (<95%) of the family Isosphaeraceae. The 16S rRNA gene sequence identity between strains was >99.

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A Gram-stain-negative, aerobic, pear to oval shaped, rosette forming bacterium with crateriform structures well distributed on the cell surface designated as strain JC647 was isolated from a sponge specimen belonging to the genus Spongia. Strain JC647 reproduces through budding. Strain JC647 shared highest 16S rRNA gene sequence identity of 99.

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A yellow-colored, Gram-stain-positive, rod shaped, non-motile bacterium, designated as strain JC619, was isolated from the sediment of Chilika lagoon, India. Strain JC619 shows highest 16S rRNA gene sequence similarity (99.08%) with Isoptericola chiayiensis KCTC 19740 followed by Isoptericola halotolerans KCTC 19046 (98.

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An endospore producing, strict aerobic, Gram-stain-positive, orange-colored colony forming bacterium designated as strain JC1013 was isolated from an orange pond near a solar saltern of Tamil Nadu, India. Phylogenetic analysis of the 16S rRNA gene sequences indicated that strain was affiliated to the family Bacillaceae of the phylum Firmicutes. Strain showed highest 16S rRNA gene sequence identity of 98.

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A novel strain of Planctomycetes, designated JC670, was isolated from a high altitude (~ 2900 m above sea level) soil sample collected from Garhwal region in the Western Himalaya. Colonies of this strain were observed to be light pink coloured with spherical to oval shaped cells having crateriform structures distributed all over the cell surface. The cells divide by budding.

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A Gram-stain-negative, aerobic, non-motile, salt- and alkali-tolerant, pear to oval shaped, rosette-forming, white coloured, bacterium, designated as strain JC646, was isolated from a sediment sample collected from Chilika lagoon, India. Strain JC646 reproduced through budding, grew well at up to pH 9.0 and tolerated up to 7 % NaCl.

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Strain JC651 was isolated from a sediment sample collected from Chilika lagoon, which is one of the world's most important brackish water lakes with estuarine characteristics. Colonies of this strain are light pink and cells are Gram-stain negative, spherical to pear shaped and form rosettes. Strain JC651 grows well up to pH 9.

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A Gram-stain-negative, non-motile, coccoid-shaped, catalase- and oxidase-positive, non-denitrifying, neutrophilic bacterium designated as strain JC501 was isolated from an epiphytic rhizosphere of an orchid, , growing in the Western Ghats of India. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain JC501 belonged to the genus and had the highest levels of sequence identity with KKL-A5 (98.9 %), WPAn02 (97.

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A phototrophic bacterium, designated as strain JA983, was isolated from a freshwater pond in Gujarat, India. The strain was yellowish brown, catalase- and oxidase-positive, rod-to-oval shaped, Gram-stain-negative and motile. Growth was observed at 20-35 °C.

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The genus is taxonomically well studied, and some members are model organisms. However, this genus is comprised of a heterogeneous group of members. 16S rRNA gene-based phylogeny of the genus indicates a motley assemblage of anoxygenic phototrophic bacteria (genus ) with interspersing members of other genera (chemotrophs) making the genus polyphyletic.

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A Gram-stain-negative, motile, alkali-tolerant, swollen-rod shaped, reddish brown coloured, phototrophic bacterium designated as strain JA980, was isolated from freshwater sampled at Umiam lake, Shillong, India. Strain JA980 grew well up to pH 9.0.

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An oval- to rod-shaped, motile, Gram-stain-negative, oxidase-positive, catalase-negative, pink-coloured phototrophic bacterium (designated as strain JA968) was isolated from an estuary near Pata, Gujarat, India. Cells had an intracytoplasmic membrane architecture as lamellae and divided by budding. Strain JA968 had bacteriochlorophyll- and spirilloxanthin series carotenoids as photosynthetic pigments.

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Article Synopsis
  • Strain JC507 is a Gram-stain-negative, rod-shaped, and non-motile bacterium isolated from yeast, showing positive oxidase and catalase reactions.
  • Genetic analysis showed it closely relates to NBRC 14944 (98.7% similarity), indicating it belongs to a new species within its genus.
  • Its unique fatty acid profile and distinct genomic characteristics propose the name “sp. nov.” for this new species, with JC507 identified as the type strain.
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Exploration of renewable energy sources is an imperative task in order to replace fossil fuels and to diminish atmospheric pollution. Hydrogen is considered one of the most promising fuels for the future and implores further investigation to find eco-friendly ways toward viable production. Expansive processes like electrolysis and fossil fuels are currently being used to produce hydrogen.

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Article Synopsis
  • All three domains of life rely on a structured plasma membrane for survival, with hopanoids playing a crucial role in bacterial membrane stability similar to cholesterol in eukaryotes.
  • A study on the genome-wide transcriptome of Rhodopseudomonas palustris showed that hopanoid deficiency upregulated 299 genes and downregulated 306 genes, affecting functions such as chemotaxis and membrane transport.
  • Disruption of hopanoid synthesis could lead to new antimicrobial strategies, particularly by targeting lipid synthesis to compromise membrane integrity.
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An electrogenic bacterium was isolated from a marine coral, designated as strain JC435 and its taxonomic status examined by using a polyphasic approach. Results from the 16S rRNA gene sequence study showed that the isolate belonged to the genus Rhodococcus and formed a cluster with Rhodococcus ruber KCTC 9806 (99.5 % 16S rRNA gene sequence similarity) and Rhodococcus aetherivorans JCM 14343 (99.

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A Gram-stain-negative, rod-shaped, non-motile, aerobic bacterium was isolated from a sediment sample obtained from a wild ass sanctuary in Gujarat, India. The strain designated JC490 was oxidase- and catalase-positive. The 16S rRNA gene sequence analysis and sequence comparison data indicated that strain JC490 was a member of the genus Chryseobacterium and was closely related to Chryseobacterium jeonii AT1047 (96.

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Metagenome analysis of coastal marine habitats of Gujarat, India indicated the presence of twelve novel putative lineages of spirochaetes. Out of which a strain designated JC444 representing a novel putative lineage seven was isolated and characterized based on a polyphasic taxonomic approach. Strain JC444 was helical, Gram-stain-negative, obligate anaerobe, catalase and oxidase negative.

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Three strains (JA826T, JA912T and JA913), which were yellowish brown colour, rod to oval shaped, Gram-stain-negative, motile, phototrophic bacteria with a vesicular architecture of intracytoplasmic membranes, were isolated from different pond samples. The DNA G+C content of the three strains was between 64.6 and 65.

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Strain JA878T was purified from a photoheterotrophic enrichment obtained from a sediment sample of a brown pond near Nari Salt Pan, Bhavnagar, Gujarat, India. Cells of the isolate were coccoid, motile by means of single polar flagellum and Gram-stain-negative. The internal photosynthetic membrane architecture was vesicular.

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Metabolomic study of electrogenic bacteria is a necessity to understand the extent of complex organic matter degradation and to invent new co-culture techniques to achieve complete degradation. In this study, we have subjected Alkanivorax xenomutans (KCTC 23751; NBRC 108843), a bacterium capable for biodegradation of complex hydrocarbons, to oxic and anoxic conditions in a three chambered microbial fuel cell. In an attempt to understand the molecular mechanisms during the electrogenic processes of A.

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