Interaction studies of lead(II) ion with cyclic β-(1→3),(1→6) glucans extracted from Bradyrhizobium japonicum based on 'chelation enhanced fluorescence' (CHEF) effect.

Cyclic β-(1→3),(1→6) glucans (CBGs) isolated from Bradyrhizobium japonicum bacteria are the periplasmic oligosaccharides having cyclic structures. This paper presents the isolation of CBGs from the bacteria cultured using optimized medium that improved yields to 350-450 mg per gram of cellular dry weight along with analytical interaction with lead(II) ions in the range 33.0-2.