The cultivation medium of has an effect on the contents of its main medicinal components, but the specific mechanism is still unclear. In this study, the callus, seedlings, rhizomes, and leaves of were sequenced for the PacBio SMRT. The 2-year-old stems were selected for the Illumina sequencing and metabolome sequencing to analyze the genetic mechanism of metabolic differences under different epiphytic patterns.
View Article and Find Full Text PDFBackground: has been widely used in the pharmacology industry. To effectively produce the secondary metabolites through suspension cultured cells of , it is important to exploring the full-length transcriptome data and the genes related to cell growth and chemical producing of all culture stages. We applied a combination of Real-Time Sequencing of Single Molecule (SMRT) and second-generation sequencing (SGS) to generate the complete and full-length transcriptome of suspension cultured cells.
View Article and Find Full Text PDFAuxin/Indole-3-Acetic Acid () genes are involved in auxin signaling pathway and play an important role in plant growth and development. However, many studies focus on gene families and much less known in . In this study, a total of 27 genes (BsIAA1-27) were cloned from the transcriptome of .
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