Publications by authors named "Cesar O Munoz"
Histoplasmosis is considered one of the most important endemic and systemic mycoses worldwide. Until now few molecular techniques have been developed for its diagnosis. The aim of this study was to develop and evaluate three real time PCR (qPCR) protocols for different protein-coding genes (100-kDa, H and M antigens) using an animal model.
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- * Seven PCR techniques were tested, with four demonstrating the ability to identify very low amounts of fungal DNA, achieving an overall sensitivity of 86% and a specificity of 100%.
- * Results indicated that while all labs successfully amplified the DNA, real-time PCR protocols showed high reliability and potential for clinical use with no false positives or cross-reactions noted.