Publications by authors named "Cesar La Torre Garay"
How proteins are targeted to lipid droplets (LDs) and distinguish the LD surface from the surfaces of other organelles is poorly understood, but many contain predicted amphipathic helices (AHs) that are involved in targeting. We have focused on human perilipin 4 (Plin4), which contains an AH that is exceptional in terms of length and repetitiveness. Using model cellular systems, we show that AH length, hydrophobicity, and charge are important for AH targeting to LDs and that these properties can compensate for one another, albeit at a loss of targeting specificity.
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- Aft1 and KlAft are yeast transcription activators that control iron-uptake genes but bind to different DNA sequences (TGCACCC for Aft1 and PuCACCC for KlAft).
- The study explores a nonconserved region in their DNA-binding domains, which is crucial for Aft1's function and for S. cerevisiae's growth under iron-limited conditions.
- By swapping these regions between Aft1 and KlAft, the research shows that the Aft1 region allows KlAft to bind to Aft1’s DNA site, indicating evolved changes in the DNA-binding specificity that affect iron regulation.