Rationale: The development of simplified procedures for isolating high-mass alkanes present in crude oils is described. The new procedures, which bypass the sample recovery step with hot toluene in the conventional alkane-isolation procedure, also provide an effective sample preparation route, prior to analysis by matrix-assisted laser desorption/ionisation mass spectrometry (MALDI-MS).
Methods: Urea-alkane adducts are formed by mixing sample and urea solutions on chromatographic paper or silica-coated plates.