Comparison of the Influence of Different Nucleic Acid Extraction Assays on the Sensitivity of -Specific Real-Time PCR.

For the molecular diagnosis of Chagas disease by real-time PCR (polymerase chain reaction), optimization of diagnostic accuracy is desirable. The detection limit of real-time PCR assays for the diagnosis of in human serum is affected by various influences including the choice of the nucleic acid extraction assay. In this study, three nucleic acid extraction assays were compared regarding their influence on the sensitivity of a -specific real-time PCR with 62 reference sera containing target DNA (deoxyribonucleotide acid).