Multiple phosphorylable sites in the Zaire Ebolavirus nucleoprotein evidenced by high resolution tandem mass spectrometry.

The 739-amino-acid nucleoprotein (NP) of Zaire Ebolavirus (ZEBOV) plays a key role in Ebola virion formation and replication. A stable HEK-293 cell line capable of producing an N-ter 6His-tagged recombinant form of NP - ZEBOV was created. Production of this protein was triggered in batch culture using microcarriers.

Use of a real-time polymerase chain reaction thermocycler to study bacterial cell permeabilization by antimicrobial peptides.

Antimicrobial peptides are good leads to develop new antibiotics, but knowledge of their mode of action is a prerequisite. Destruction of the microbial membranes through a detergent-like mechanism is one of these modes of action. This is usually studied by using a fluorescent nucleic acid stain such as SYTOX Green, which is impermeable to living cells.