Observations that suggest a contribution of altered dermal papilla mitochondrial function to androgenetic alopecia.

Androgenetic alopecia (AGA) is a prevalent hair loss condition in males that develops due to the influence of androgens and genetic predisposition. With the aim of elucidating genes involved in AGA pathogenesis, we modelled AGA with three-dimensional culture of keratinocyte-surrounded dermal papilla (DP) cells. We co-cultured immortalised balding and non-balding human DP cells (DPCs) derived from male AGA patients with epidermal keratinocyte (NHEK) using multi-interfacial polyelectrolyte complexation technique.

Artificial Microniche Array with Spatially Structured Biochemical Cues.

We present here a method to create arrays of microcavities that can be differentially coated on their bottom, side, and top with different proteins. These cavities range in size from single cell to multicellular aggregate. We provide detailed protocols to create such arrays with some variations using different materials and different coating proteins.