Screening of Bacterial Quorum Sensing Inhibitors in a LuxR-Based Synthetic Fluorescent Biosensor.

A library of 23 pure compounds of varying structural and chemical characteristics was screened for their quorum sensing (QS) inhibition activity using a synthetic fluorescent biosensor that incorporates a modified version of lux regulon of . Four such compounds exhibited QS inhibition activity without compromising bacterial growth, namely, phenazine carboxylic acid (PCA), 2-heptyl-3-hydroxy-4-quinolone (PQS), 1-2-methyl-4-quinolone (MOQ) and genipin. When applied at 50 µM, these compounds reduced the QS response of the biosensor to 33.

An investigation of the interactions between an E. coli bacterial quorum sensing biosensor and chitosan-based nanocapsules.

We examined the interaction between chitosan-based nanocapsules (NC), with average hydrodynamic diameter ∼114-155nm, polydispersity ∼0.127, and ζ-potential ∼+50mV, and an E. coli bacterial quorum sensing reporter strain.