Effect of anti-adhesive supplement doses on sperm motility characteristics in the European whitefish (Coregonus lavaretus).

Sperm adhering to glass slides is one of the main problems during fish sperm motility analyses with CASA systems. To mitigate this, albumin is the supplement added most frequently to activating solutions. However, there is no data on the use of supplements other than albumin (in various concentrations) in analyses of European whitefish (Coregonus lavaretus) sperm motility.

Effect of Different Activation Solutions and Protein Concentrations on Ide () Sperm Motility Analysis with a CASA System.

The purpose of this study was to compare four activation solutions (AS)-Woynarovich, Lahnsteiner, Kucharczyk, and Perchec-with the addition of 0.5% bovine serum albumin (BSA) for ide () sperm activation and analysis with a CASA system. It was found that ide sperm can be activated using each AS within a pH range of 7.

Embryonic Development and Survival of Siberian Sturgeon × Russian Sturgeon ( × ) Hybrids Cultured in a RAS System.

The aim of this study was to describe the selected stages of embryonic development in Siberian sturgeon () and hybrids of Siberian sturgeons and Russian sturgeons ( × ). For this purpose, embryos representing nine distinct developmental stages (stage 1-2.0 hpf, stage 2-5.

Ide (Leuciscus idus) sperm short-term storage: Effects of different extenders and dilution ratios.

In cyprinids, Tyrode's (TLP) and Volckaert's (VRT) solutions are the most frequently used extenders for short-term sperm storage. The effectiveness of TLP and VRT on ide (Leuciscus idus) sperm short-term storage was analyzed using a × 4 (sperm:extender) dilution ratio over 48 h. A × 4 (1:3) dilution ratio was compared to a × 10 (1:9) dilution ratio for ide sperm storage using TLP supplemented with antibiotics and was tested for a 14-day period.

Comparison of responses to artificial spawning of ruffe (Gymnocephalus cernua) specimens captured from their natural habitat to those produced in cultured conditions.

Although ruffe (Gymnocephalus cernua) are widely distributed in Eurasia, in some regions (i.e., North America) ruffe are considered an invasive species.

Comparison of artificial spawning effectiveness of hCG, CPH and GnRHa in combination with dopamine inhibitors in a wild strain of ide Leuciscus idus (L.) in hatchery conditions.

Optimization of artificial reproduction, by increasing the survival rate of embryos and hatching rate, is of major importance for reducing genetic diversity, especially in fish captured from their natural habitat that subsequently spawn in hatcheries. The artificial reproduction of ide, Leuciscus idus (L.) was conducted in controlled conditions.

A Comparison of Pneumatic and Hand Stripping of Whitefish () Eggs for Artificial Reproduction.

We describe the technique of pneumatic stripping of whitefish () eggs with the use of oxygen, nitrogen, and air. Eggs obtained via the traditional method (by pressing the abdominal surfaces) served as a control group. It was established that the gas flow rate during pneumatic stripping should not exceed 0.

Effect of different treatment agents and post-treatment latency times on spermiation stimulation of northern pike (Esox lucius) under controlled conditions.

The effect of different treatment agents, namely, carp pituitary homogenate (CPH), Ovaprim ([D-Arg, ProNEt]-sGnRH + domperidone) and a dopamine-receptor antagonist (metoclopramide), on the stimulation of northern pike (Esox lucius) spermiation was tested under controlled conditions. To carry out the experiment, males (n = 84) were divided into four groups: control (n = 21); CPH (n = 21); Ovaprim (n = 21); metoclopramide (n = 21). The control group was given 0.

Sperm quality in fish: Determinants and affecting factors.

Fish sperm quality assessment is helpful for optimizing production and for monitoring the environmental state. Sperm can be monitored relatively easy and, to date, various analyses have been applied and proven to be helpful in this task. Among them, sperm motility parameters such as sperm speed are one of the main performance traits during assisted fish reproduction.

Use of ultrasonography to determine sex in sexually immature European river lamprey Lampetra fluviatilis (L.).

In this study, there was the first attempt to sex immature European river lampreys Lampetra fluviatilis, classified as Agnatha using ultrasonography. This species starts a spawning migration from seas to rivers in the autumn and reproduction is initiated in the late spring. It is recommended to collect breeders soon after the beginning of the spawning migration, however, to date no method has been developed for distinguishing the sex of individuals during this developmental period.

Effectiveness of the Air Stripping in Two Salmonid Fish, Rainbow Trout (Oncorhynchus Mykiss) and Brown Trout (Salmo Trutta Morpha fario).

Egg collection is one of the most crucial procedures during fish reproduction in salmonid hatcheries. Classic methods involve the use of hand massage on fish abdomens to expel the eggs. An alternative method uses the pressure of gas injected into the body cavity, which causes the subsequent release of the eggs.

Effect of carp pituitary homogenate (CPH) and sGnRHa (Ovaprim) on northern pike (Esox lucius) spermiation stimulation and its effect on quantity and quality of sperm.

The effect of carp pituitary homogenate (CPH, n = 7) at a dose of 2.0 mg kg and (D-Arg, ProNET)-sGnRH + domperidone (Ovaprim, n = 7) at a dose of 0.5 ml kg in northern pike (Esox lucius) sperm maturation under controlled conditions was examined.

Optimisation of sodium and potassium concentrations and pH in the artificial seminal plasma of common carp Cyprinus carpio L.

The effect of sodium and potassium concentrations as well as optimal pH on the motility of common carp Cyprinus carpio L. sperm during short-term storage in artificial seminal plasma (ASP) was investigated. Sperm was collected from individual males (n = 5) and each sample diluted tenfold (1:9) in ASP (sperm:extender) containing 2 mM CaCl, 1 mM MgSO and 20 mM Tris at pH 8.

Evidence of the sterility of allotetraploid Cobitis loaches (Teleostei, Cobitidae) using testes ultrastructure.

The diploid-polyploid populations of Cobitis distributed in Poland are usually composed of the spined loach Cobitis taenia or, less often, the Danubian loach C. elongatoides and their triploid (females) and tetraploid hybrids (females and males). The aim of this study was to determine whether tetraploid males participate in the reproduction process by analyzing their testis ultrastructure and the process of spermatogenesis in comparison with diploid males of both parental species.

Multiple collections of common carp Cyprinus carpio L. semen during the reproductive period and its effects on sperm quality.

Multiple collections of semen during the reproductive period of the common carp Cyprinus carpio L. were used to analyse changes in semen quality. Semen collection was performed on June 1 (first collection), 12 (second collection), and 19 (third collection) from individual males (n=11) by gentle abdominal massage.

Effects of hCG and salmon gonadoliberine analogue on spermiation in the Eurasian perch (Perca fluviatilis).

This study analysed (i) the effect of human chorionic gonadotropin (hCG) and salmon gonadoliberine analogue (sGnRHa) on the effectiveness of induction of spermiation and (ii) the effect of latency time following the application of those spawning agents on the quantity and quality of the sperm of Eurasian perch, Perca fluviatilis, obtained during out-of-season spawning. For this study, pond-reared fish were used which had been acclimated to the controlled conditions. Three groups were distinguished which were treated with either saline (0.

Serine-like proteolytic enzymes from common carp Cyprinus carpio L. seminal plasma are able to degrade sperm proteins.

The application of zymography, with sperm proteins as a substrate, allowed for the first time the visualisation of two serine proteinases with a molecular weight of 76 and 163kDa from common carp Cyprinus carpio L. seminal plasma. Twenty four hours of incubation in a development solution with a pH of 7.

The effect of different ambient temperatures on river lamprey (Lampetrafluviatilis) egg and sperm production under controlled conditions.

Reproductive performance (ovulation/spermation rate and relative fecundity of females) of adult river lamprey (Lampetrafluviatilis) was compared among adults held under three different controlled thermal regimes (7, 10 and 14°C). The quantity of semen (volume of semen, sperm concentration, total sperm production and total number of sperm) and the weight of the eggs as well as the semen quality (sperm motility, seminal plasma osmolality and pH, sperm pH and total protein content) were determined. Housing temperature had no apparent effect on quality or quantity of eggs produced, but did influence time of ovulation.

Substrate specificity of proteolytic activity in the testes fluid and seminal plasma of the common carp Cyprinus carpio.

Substrate specificity in the seminal plasma and testes fluids of the common carp Cyprinus carpio was determined using gelatin, casein, albumin and haemoglobin. Proteolytic profiles of the testes and seminal plasma were compared. Different ranges of pH (5·5-9·5) and temperature (4-37° C) were used during incubations of seminal plasma proteinases.

Application of dopaminergic antagonist: Metoclopramide, in reproduction of crucian carp Carassius carassius (L.) under controlled conditions.

The effectiveness of Ovopel (two pellets/kg body weight), a complex of [D-Ala(6) Pro(9)NEt-mGnRH] and dopamine antagonist (DA e.g. Metoclopramide, MET), was compared with the application MET alone at the dose of 20mg/kg body weight in spawning crucian carp Carassius carassius (L.

Inhibition of β-N-acetylglucosaminidase by acetamide affects sperm motility and fertilization success of rainbow trout (Oncorhynchus mykiss) and Siberian sturgeon (Acipenser baerii).

β-N-Acetylglucosaminidase (β-NAGase) is an enzyme found in the sperm acrosome of numerous animal species including fish. Fish spermatozoa differ in their morphology including acrosome or acrosomeless aquasperm in chondrostean (e.g.

Quality and quantity of smelt (Osmerus eperlanus L.) sperm in relation to time after hormonal stimulation.

The effect of Ovaprim (salmon GnRH analogue and a dopamine antagonist) treatment on the quantity and quality of smelt (Osmerus eperlanus L.) sperm was studied in relation to time after hormonal stimulation. Sperm was obtained at 0, 24, 48 and 72 h after treatment (n=13/each time point).

Artificial reproduction of wild and cultured barbel (Barbus barbus, Cyprinidae) under controlled conditions.

The aim of this work was to compare the effects of controlled reproduction of cultured and wild common barbel, Barbus barbus (L.). Preparations containing different GnRH analogues and dopamine receptor antagonists (Ovopel, Ovaprim) as well as human chorionic gonadotropin (hCG) (in the case of cultured fish) were applied and their influence on ovulation, spermiation and quality of gametes obtained was determined.