Integral genomic description of bla-harbouring plasmids recovered from Enterobacterales in Argentina.

Objective: To characterise four bla-harbouring plasmids recovered in Enterobacterales isolated in Argentina.

Methods: DNA was sequenced by Illumina and Oxford Nanopore Technologies, assembled using Unicycler, analysed using PlasmidFinder, MOB-Typer, IslandViewer4, and Resfinder, and visualised by Proksee and Clinker. bla-harbouring plasmids were compared with similar deposited plasmids using PLSDB.

Genomic characterization of carbapenemase-producing Klebsiella pneumoniae ST307 revealed multiple introductions in Buenos Aires, Argentina.

Objectives: To describe at genomic level nine carbapenemase-producing Klebsiella pneumoniae ST307 (Kp-ST307) clinical isolates recovered in Buenos Aires during 2017 to 2021, investigating their resistome, virulome, and phylogeny.

Methods: Antimicrobial susceptibility was determined according to Clinical and Laboratory Standards Intitute (CLSI). Genomic DNA was sequenced by Illumina MiSeq and analysed using SPAdes, PROKKA, and Kleborate.

Comparison of two MALDI-TOF MS systems for the identification of clinically relevant anaerobic bacteria in Argentina.

The aim of this study was to compare the performance of two MALDI-TOF MS systems in the identification of clinically relevant strict anaerobic bacteria. The 16S rRNA gene sequencing was the gold standard method when discrepancies or inconsistencies were observed between platforms. A total of 333 isolates were recovered from clinical samples of different centers in Buenos Aires City between 2016 and 2021.

Genomic characterization of bla-harbouring plasmids recovered from Pseudomonas aeruginosa.

Objectives: To the best of our knowledge, no genomic descriptions of bla-harbouring plasmids are available in literature so far. The aim of this study was to describe the genomic features of three bla-harbouring plasmids recovered from Pseudomonas aeruginosa isolated in Argentina in different periods.

Methods: bla-harbouring plasmids from three clinical P.

Promoting Science Literacy and Awareness across the Globe: the Role of Scientists as Science Ambassadors.

Science literacy has many personal and societal benefits that allows for better informed decision-making. Although the importance of science literacy is recognized globally, there are many challenges associated with its promotion. Scientists are more frequently engaging with nonscientific audiences through public outreach activities and with increasing support from institutions and professional societies.

Characterization of a Clostridioides difficile ST-293 isolate from a recurrent infection in Argentina.

Clostridioides difficile is an opportunistic spore-forming pathogen responsible for antibiotic-associated diarrhea in humans. C. difficile produces two main toxins: TcdA and TcdB as well as a third toxin named binary toxin (CDT) that is also involved in virulence.

Outbreak of Klebsiella pneumoniae ST11 Resistant To Ceftazidime-Avibactam Producing KPC-31 and the Novel Variant KPC-115 during COVID-19 Pandemic in Argentina.

We describe an outbreak of Klebsiella pneumoniae sequence type 11 (ST11) producing KPC variants resistant to ceftazidime-avibactam. Six patients hospitalized in the intensive care unit (mostly due to critical COVID pneumonia) presented infection or colonization by this bacterium. They had several comorbidities and required mechanical ventilation, central venous catheters, and urinary catheters.

Signals of adaptation to agricultural stress in the genomes of two European bumblebees.

Human-induced environmental impacts on wildlife are widespread, causing major biodiversity losses. One major threat is agricultural intensification, typically characterised by large areas of monoculture, mechanical tillage, and the use of agrochemicals. Intensification leads to the fragmentation and loss of natural habitats, native vegetation, and nesting and breeding sites.

Emergence and clonal expansion of Klebsiella pneumoniae ST307, simultaneously producing KPC-3 and NDM-1.

MDR Klebsiella pneumoniae ST307 is a high-risk clone, whose genetic features contribute to its adaptation to hospital environments and the human host. This study describes the emergence and clonal dissemination of K. pneumoniae ST307, recovered during November 2018 to February 2019 in a hospital in Buenos Aires city, which concurrently harbored KPC-3 and NDM-1.

Health Equity for Children and Youth With Special Health Care Needs: A Vision for the Future.

Health equity is a key pillar in supporting a future in which CYSHCN enjoy a full life and thrive, as envisioned by experts and community partners who gathered in 2019 and 2020 to develop the Blueprint for Change: Guiding Principles for a System of Services for Children and Youth With Special Health Care Needs and Their Families. However, a variety of contextual factors impact health outcomes across the life course and intergenerationally and must be addressed to achieve this goal. For example, poverty and discrimination, including by some health care professionals and systems, are important, modifiable root causes of poor health outcomes.

Characterisation of blaharbouring plasmids recovered from Pseudomonas aeruginosa ST654 and ST235 high-risk clones.

Objective: The main objectives were to describe two bla plasmids recovered from Pseudomonas aeruginosa isolates belonging to the ST654 and ST235 high-risk clones, and to compare with complete sequences of bla harbouring plasmids available in public databases.

Methods: Antimicrobial susceptibility was determined according to CLSI (Clinical and Laboratory Standards Institute) guidelines. Genomes were sequenced using an Illumina MiSeq platform, and bla plasmid sequences were achieved using MinION platform.

The dilemma of identifying Peptoniphilus species by using two MALDI-TOF MS systems.

Two commercial MALDI-TOF MS systems were used to identify 18 isolates, belonging to the Peptoniphilus genus; also the 16S rRNA sequencing identity was compared against the MALDI-TOF MS system results. Bruker Biotyper system provided higher accuracy than Vitek MS system, however, adding spectra could allow a more reliable species level identification.

Spatial and temporal patterns of genetic diversity in Bombus terrestris populations of the Iberian Peninsula and their conservation implications.

The bumblebee Bombus terrestris is used worldwide for crop pollination. Despite its positive impact on crop yield, it has become a widespread threat to biodiversity due to its interactions with local bumblebee populations. Commercial subspecies introduced to the Iberian Peninsula since the 1990s without any regulation have colonized the environment, with evidence of naturalization and introgression with the endemic subspecies Bombus terrestris lusitanicus.

An update and revision of the fauna of Morocco (Hymenoptera, Apoidea, Andrenidae) with the description of eleven new North African species.

Morocco has a diverse bee fauna, but one that has also been relatively understudied in recent years. Here a revision of the species-rich genus is presented that reveals eleven new species for science and substantially improves our understanding of North African . From Morocco, Andrena (Aciandrena) semiadesus Wood, , Andrena (Aciandrena) triangulivalvis Wood , Andrena (Campylogaster) sparsipunctata Wood , Andrena (Carandrena) hebescens Wood , Andrena (Cnemidandrena) niveofacies Wood , () Wood , Andrena (Notandrena) acutidentis Wood , Andrena (Poliandrena) breviceps Wood , and Andrena (Poliandrena) farinosoides Wood are described and their ecology is discussed.

Full characterization of an IncR plasmid harboring qnrS1 recovered from a VIM-11-producing Pseudomonas aeruginosa.

Metallo-β-lactamases (MBL) producing Pseudomonas aeruginosa isolates have been well characterized. Quinolones are commonly used in the treatment of carbapenem-resistant P. aeruginosa infections; however, data about PMQR in this species are scarce.

Unveiling introgression in bumblebee (Bombus terrestris) populations through mitogenome-based markers.

The bumblebee, Bombus terrestris, is an important pollinator commercially used on a global scale. The exported subspecies B. t.

Changing epidemiology of KPC-producing Klebsiella pneumoniae in Argentina: Emergence of hypermucoviscous ST25 and high-risk clone ST307.

Objectives: To assess the epidemiological features of 76 Klebsiella pneumoniae carbapenemase (KPC)-producing Klebsiella pneumoniae (KPC-Kp) isolates recovered from three hospitals in Buenos Aires, Argentina, during 2015-2017.

Methods: Antimicrobial susceptibilities were determined according to CLSI Clinical and Laboratoy Standards guidelines. Molecular typing of KPC-Kp was performed by pulsed-field gel electrophoresis (PFGE)-Xbal and multilocus sequence typing.

MALDI-TOF MS based procedure to detect KPC-2 directly from positive blood culture bottles and colonies.

In this study, we identified specific carbapenemase-producing isolates applying an easy and rapid protocol for the detection of mature KPC-2 β-lactamase by MALDI-TOF MS from colony and positive blood culture bottles. In addition, we evaluated the correlation of the ~11,109 Da signal as a biomarker associated with KPC-2 production. A collection of 126 well-characterized clinical isolates were evaluated (including 60 KPC-2-producing strains).

Simultaneous Carriage of and Other Antimicrobial Resistance Determinants in From Poultry.

The use of antimicrobial growth promoters (AGPs) in sub-therapeutic doses for long periods promotes the selection of resistant microorganisms and the subsequent risk of spreading this resistance to the human population and the environment. Global concern about antimicrobial resistance development and transference of resistance genes from animal to human has been rising. The goal of our research was to evaluate the susceptibility pattern to different classes of antimicrobials of colistin-resistant from poultry production systems that use AGPs, and characterize the resistance determinants associated to transferable platforms.

Spread of Clonally Related Escherichia coli Strains Harboring an IncA/C Plasmid Encoding IMP-8 and Its Recruitment into an Unrelated MCR-1-Containing Isolate.

Ten IMP-8-producing isolates were recovered from surveillance cultures of a neonatal intensive care unit; eight of the isolates were clonally related. A 168.2-kb plasmid was fully sequenced, and it corresponded to the recently described IncA/C1-ST13 plasmid.

Fast and easy detection of CMY-2 in Escherichia coli by direct MALDI-TOF mass spectrometry.

Fast typing methods for third generation cephalosporin resistance mechanisms are needed to guide appropriate treatment and prevent potential dissemination events. In this study we used a novel short and fast methodology for the identification of CMY-2 in 50 well characterized clinical isolates of E. coli by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry- MALDI-TOF MS.

Detection and molecular characterization of Clostridium difficile ST 1 in Buenos Aires, Argentina.

Thirty one C. difficile isolates recovered in 2015 were characterized. Nineteen/31 were positive for tcdA/B, among them, 4 isolates were also positive for CDT coding genes.