Publications by authors named "Cedric Abele"
Article Synopsis
- This study highlights the effectiveness of in vivo molecular staining and automated imaging to analyze mitochondrial health in various test organisms.
- A new protocol was developed using JC-1 dye to assess mitochondrial membrane potential, and 5% ethanol was identified as the best anesthetic for healthy recovery.
- The automated imaging method revealed that environmental contaminants had mitochondrial effects at lower concentrations than traditional tests, demonstrating its sensitivity and efficiency for ecotoxicological testing.
High-throughput reporter gene assays are increasingly applied to assess the potency of chemicals to alter specific cellular signaling pathways. Genetically modified reporter gene cell lines provide stable readouts of the activation of cellular receptors or transcription factors of interest, but such reporter gene assays have been criticized for not capturing cellular metabolism. We characterized the metabolic activity of the widely applied AREc32 (human breast cancer MCF-7), ARE-bla (human liver cancer HepG2), and GR-bla (human embryonic kidney HEK293) reporter gene cells in the absence and in the presence of benzo[]pyrene (BaP), an AhR ligand known to upregulate cytochrome P450 and .
View Article and Find Full Text PDFCellular uptake kinetics are key for understanding time-dependent chemical exposure in in vitro cell assays. Slow cellular uptake kinetics in relation to the total exposure time can considerably reduce the biologically effective dose. In this study, fluorescence microscopy combined with automated image analysis was applied for time-resolved quantification of cellular uptake of 10 neutral, anionic, cationic, and zwitterionic fluorophores in two reporter gene assays.
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