Publications by authors named "Ceder O"

A disturbed activity of ribonuclease (RNAase) and its natural inhibitor was found in cystic fibrosis patients (CF); Heterozygotes had normal RNAase but increased inhibitor activities; both groups compared with age-matched controls. RNAase is involved in the breakdown of RNA. In the cell it is bound to an inhibitor and thus inactive (Bardoń 1976).

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An increased activity of the glycolytic pathway was found in RBC: s from CF patients, compared to age-matched controls. Heterozygotes showed a tendency to increased activity.

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The excretions of 3,4-dihydroxyphenylalanine (DOPA) metabolites and 5-hydroxyindoleacetic acid (5-HIAA) were followed before, during, and after a marathon race in three patients with cystic fibrosis (CF) and three healthy controls. Moderately increased DOPA excretions in CFs compared with controls before, during, and after the race support the idea that there is an altered metabolism of DOPA in CF. Noradrenaline, adrenaline, homovanillic acid, and vanilmandelic acid were increased in CFs compared with controls mainly during and after the race, which might be a reflection of the heavier working intensity that the CF patients had in comparison with their healthy controls.

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Lipid metabolism was studied in three cystic fibrosis (CF) patients (18 years old) and three healthy controls (35-40 years old) who completed the New York Marathon 1984. Lipase was not detectable in plasma from two of the CF patients, but in one CF patient with a functioning pancreas and in the three controls it increased during and after the run. Cholesterol concentrations were lower in all patients than in the controls.

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Biochemical changes and endocrine responses during the New York Marathon (42195 m) were investigated in three 18-year-old male adolescents with cystic fibrosis (CF) and three healthy men who accompanied the CFs during the race. The ambient temperature was 20 degrees-28 degrees C and the relative humidity 98%-75% during the run. The CF patients, who had Shwachman scores of 60, 85 and 95 completed the run without major problems in 6.

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The purpose of this study was to compare biochemical changes and endocrine responses during an incremental maximal bicycle test in three well-trained 18-year-old patients with cystic fibrosis (CF) and in three healthy controls. Although the blood concentration at rest of the white cell count, haptoglobin, phosphorus, urea, creatinine, and uric acid were somewhat different in the two groups, the CF patients had similar biochemical changes in response to the exercise compared with the healthy men. The endocrine responses to exercise seemed to be different between the two groups with regard to changes in cortisol, growth hormone, and testosterone concentrations.

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The mean levels of activity of hexokinase, phosphofructokinase, pyruvate kinase and lactate dehydrogenase were found to be significantly higher in fibroblasts from cystic fibrosis patients than in those from age-matched controls and heterozygotes. This indicates that the activity of the glycolytic pathway is increased in fibroblasts from cystic fibrosis patients.

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The chronically pilocarpine-treated rat has been proposed as an animal model for the disease cystic fibrosis, a generalized exocrinopathy. The effect of chronic pilocarpine treatment on structure, composition, and function of the acinar cells of rat submandibular gland and pancreas was investigated by electron microscopy, X-ray microanalysis, and biochemical analysis. The morphological effects of chronic pilocarpine treatment were most pronounced in the pancreas.

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The effect of chronic treatment with cystic fibrosis (CF) fibroblast medium on rat submandibular gland and pancreas was investigated. Rats were injected for 8 days with conditioned medium from normal or CF fibroblasts. The elemental content of the acinar cells was measured by X-ray microanalysis of cryosections.

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A decreased intracellular pH of exocrine glands could be an important factor in the pathogenesis of cystic fibrosis. Metabolic acidosis was induced in rats by adding ammonium chloride to the drinking water. An increased content of both total proteins and glycoproteins was found in the submandibular glands of the treated animals.

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Some of the enzymes and metabolites of the glycolytic pathway of an animal model for cystic fibrosis (the chronically reserpine-treated rat) were investigated. The activities of the enzymes phosphofructokinase (P less than 0.002), enolase (P less than 0.

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The flow rate, the activity of ribonuclease (RNAase) and the concentration of protein were determined in whole saliva and in parotid and submandibular saliva from patients with cystic fibrosis (CF) and from healthy controls, both before and after stimulation of the salivary secretion. Lower flow rates were found in all types of saliva from CF-patients than in control saliva. Increased activity of RNAase was found in CF saliva, both before and after stimulation of the secretion.

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Alkaline acid- and thermo-stable ribonuclease (RNAase) was assayed in whole mixed saliva from controls and from cystic fibrosis (CF) heterozygotes and homozygotes. There was a significant difference in salivary RNAase activity between control adults (n = 99) and CF heterozygotes (n = 77) (12.6 +/- 0.

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The biochemical composition of saliva secreted by healthy persons and by heterozygotes and homozygotes of cystic fibrosis at rest and by healthy persons subjected to aerobic or anaerobic effort were compared. In the saliva from cystic fibrosis homozygotes at rest substantial increases of the activity of ribonuclease (p less than 0.001) and of the concentrations of protein (p less than 0.

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The effect of culture medium from fibroblast cultures of cystic fibrosis (CF) patients and healthy controls on the elemental composition of fibroblasts was investigated by X-ray microanalysis. Exposure of normal fibroblasts to culture medium from CF fibroblasts caused an increase in calcium level. Exposure of CF fibroblasts to culture medium from normal cells caused an increase of the sodium content of CF cells to approximately normal levels; the calcium level of the CF fibroblasts, however, remained abnormally high.

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The diagnosis of cystic fibrosis (CF) homozygotes and heterozygotes and of individuals without the CF gene, based on differences in the thermal stability of acid phosphatase and alpha-mannosidase, is reported. The residual activities at 36.5 degrees C and 41.

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Cystic fibrosis (CF) is the most common severe, autosomal recessive disease in Caucasians. The main clinical symptoms are all related to exocrine gland disturbances and include obstructive lung disease, pancreatic insufficiency and increased sweat electrolytes. In the present investigation fibroblasts from CF homozygotes were studied by X-ray microanalysis and were shown to have an increased calcium and a decreased sodium content, compared with fibroblasts from controls.

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The results of more than eight years of screening for cystic fibrosis (CF), utilizing the albumin concentration in meconium, are reported. Over 28 000 meconium samples were tested, over 24 000 of them by the Boehringer-Mannheim strip test, and all samples considered as showing a trace or positive result were followed up by an immunological technique. Eight-three meconium samples contained more than 20 g albumin/kg dry weight meconium and five of these were from children who were found to have CF.

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The chronically reserpinized rat has been suggested as an animal model for cystic fibrosis. X-ray microanalysis of thick and thin cryosections was carried out to assess elemental redistribution in the submandibular glands and the pancreas of reserpinized rats at the cellular and subcellular level. In the submandibular gland of reserpinized rats, calcium and magnesium concentrations were significantly elevated.

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The elemental composition of cultured fibroblasts from trisomy patients, cystic fibrosis patients, and from healthy controls was compared by means of electron probe X-ray microanalysis. Significantly higher relative Ca concentrations (relative to phosphorus) and higher Ca/K ratios, were found in both trisomy and CF fibroblasts. Significantly lower relative Na concentrations and lower Na/K ratios were only found in CF fibroblasts; the Na content of trisomy fibroblasts was normal.

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As previously reported a cystic fibrosis factor (CFF) is associated with the dyskinetic ciliary motion induced by serum and cell culture medium from patients with cystic fibrosis (CF). In this study a sensitive, standardization method, for the photoelectric recording of mucociliary activity was used to examine the effect of sera and media from cell cultures taken from patients with CF and healthy controls, on the mucociliary activity of rabbit trachea in vitro. No signs of decreased mucociliary activity were observed and electron microscopy showed normal ultrastructure and orientation of cilia.

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The ultrastructure of cultured fibroblasts of patients with cystic fibrosis (CF) was investigated and compared with control cultures. CF-fibroblasts showed a significantly increased number of pinocytotic vesicles along the plasma membrane. Other differences in ultrastructure between CF-fibroblasts and controls could not be observed.

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The enzyme activities of alpha-fucosidase (pH 4.0 and pH 5.5), alpha-galactosidase, beta-galactosidase, alpha-glucosidase (pH 4.

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