Flaviviruses in migratory passerines during spring stopover in a desert oasis.

Bird migration has long been hypothesized as the main mechanism for long-distance dispersal of flaviviruses, but the role of migratory birds in flaviviruses spillover is not well documented. In this study, we investigated the eco-epidemiology of West Nile virus (WNV) and Usutu virus (USUV) in trans-Saharan passerines during their spring stopover in southern Tunisian oases. To do, we combined oral swab analysis and serological tools to assess whether migratory birds could be reaching these stopover sites while infectious or have been previously exposed to viruses.