Rapid detection of KPC-producing Enterobacterales by using a modified Carba NP test with imipenem/relebactam.

Introduction: Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam.

Material And Methods: The test performance was evaluated in a random selection of 160 previously molecularly characterized clinical isolates carrying the 110 bla, 1 bla, 12 bla, 4 bla, 3 bla and 42 bla genes. The proposed method relies on the detection of imipenem hydrolysis in an imipenem/relebactam antibiotic solution and subsequent visual interpretation by color change.

Rapid detection of KPC-producing Enterobacterales by using a modified Carba NP test with imipenem/relebactam.

Introduction: Here, we propose a novel modified Carba NP test for detecting KPC-producing Enterobacterales using imipenem/relebactam.

Material And Methods: The test performance was evaluated in a random selection of 160 previously molecularly characterized clinical isolates carrying the 110 bla, 1 bla, 12 bla, 4 bla, 3 bla and 42 bla genes. The proposed method relies on the detection of imipenem hydrolysis in an imipenem/relebactam antibiotic solution and subsequent visual interpretation by color change.

Rapid direct detection of carbapenemase-producing Enterobacteriaceae in clinical urine samples by MALDI-TOF MS analysis.

Objectives: Development of an automated MALDI-TOF MS-based method for the rapid, direct detection of carbapenemase-producing Enterobacteriaceae in clinical urine samples within 90 min of sample reception.

Methods: A total of 3041 urine samples were processed by flow cytometry, and a cut-off value of ≥1.5 × 10 5 bacteria/mL was used to select samples for the study.