Antibiofilm Efficacy of Calcium Silicate-Based Endodontic Sealers.

Background: Using endodontic sealers with long-term antimicrobial properties can increase the success of endodontic treatment. This study aimed to assess the antimicrobial activity over time of two calcium silicate (CS)-based sealers, AH Plus Bioceramic and BioRoot RCS, and to compare them with an epoxy resin-based sealer, AH Plus Jet, against mature polymicrobial biofilms grown on human radicular dentin.

Methods: The antimicrobial activity of the sealers was tested using a direct contact test after 1 and 6 weeks of contact with the biofilms.

Understanding dental caries as a non-communicable and behavioral disease: Management implications.

New paradigms in caries conceptualization have emerged during the last decades, leading to intense debate and discussion on how to approach the disease, both from a preventive and a therapeutic perspective. Among many new ideas, research discoveries and technologies, one major concept can be highlighted that created a deep frontier between the old and the new paradigm in caries conceptualization; the non-communicable nature of the disease, firmly associated with behaviors and lifestyles. This article synthetizes the conceptual construction of dental caries as a non-communicable disease (NCD) based on the current evidence and discusses the appropriate management of the disease in this context.

Detection of Cavitated Proximal Carious Lesions in Permanent Teeth: A Visual and Radiographic Assessment.

Detection of proximal carious lesions involves the combination of clinical and radiographic methods, both with inherent difficulties. The present cross-sectional study is aimed at estimating the prevalence of cavitation in proximal carious lesions, based on a direct clinical assessment of previously detected radiographic lesions, in permanent molars and premolars. Proximal dental surfaces were radiographically evaluated using the ADA coding system and cavitation was determined through clinical visual examination of the surfaces after separation with elastomeric bands.

Microcavitated (ICDAS 3) carious lesion arrest with resin or glass ionomer sealants in first permanent molars: A randomized controlled trial.

Objectives: Although there is some consensus that carious lesions in early stages (non-cavitated) could be treated using sealants, neither the type of materials nor their use in lesions with localized enamel breakdown (microcavitated) has been reported To compare the efficacy of resin or glass ionomer (GI) sealants in arresting microcavitated carious lesions (ICDAS 3) in first permanent molars.

Materials And Methods: A double-blinded randomized controlled clinical trial was conducted in 41 healthy 6 to 11-year-old children. At the baseline examination, each subject had at least one carious lesion classified as ICDAS 3 on the first permanent molar.

Anticaries activity of egg ovalbumin in an experimental caries biofilm model on enamel and dentin.

Objectives: Limited evidence suggests a putative inhibitory effect of dietary proteins on demineralization during the carious process. The aim was to explore a potential anticaries activity of the egg protein ovalbumin on a relevant in vitro approach.

Materials And Methods: Biofilms of Streptococcus mutans UA159 were formed on saliva-coated enamel and dentin bovine slabs.

Evidence-based strategies for the minimally invasive treatment of carious lesions: Review of the literature.

Resulting in a high economic and biological cost, the traditional therapeutic approach to carious lesion management is still largely restorative. Minimally invasive (MI) treatment offers an attractive alternative to managing carious lesions in a more conservative and effective manner, resulting in enhanced preservation of tooth structure. The aim of this review was to summarize the evidence behind several MI alternatives for carious lesion management, including the use of sealants, infiltration, atraumatic restorative treatment (ART), and selective carious tissue removal (e.

Longer Retention Time of Fluoridated Varnishes Enhances Enamel Remineralisation In Vitro.

Purpose: Widely used in caries prevention, fluoridated varnish (FV) is also capable of remineralising carious lesions. However, the retention time of FV needed on enamel to ensure the maximum benefit is unclear. This study aimed to determine whether an increase in the retention time of 5% sodium fluoride (NaF) varnishes on enamel carious lesions enhances remineralisation.

Stability of chemically available fluoride in Chilean toothpastes.

Background: Storage time may reduce the amount of soluble fluoride (F) in toothpastes. Although we previously studied the type and concentration of F in fresh samples of commercial Chilean toothpastes, their stability was not determined.

Aim: To evaluate the stability of soluble F in Chilean toothpastes after 1 year of storage.

In situ anticariogenic activity of free fatty acids after sucrose exposure to oral biofilms formed on enamel.

Purpose: To test whether the effect of sucrose on enamel is hampered by immediate free fatty acids exposure to the oral biofilm formed under a highly cariogenic environment, in situ.

Methods: A split-mouth, cross-over and double-blind in situ experiment was carried out with 11 volunteers who wore palatal appliances containing bovine enamel slabs. In two 15-day phases, volunteers dripped 20% sucrose onto the slabs eight times per/day for 5 minutes followed by 5 additional minutes with suspensions of 10 mM oleic, linoleic or stearic acid or sucrose (caries-positive control), four times/day.

Sealing Dentin Caries with Resin-Modified Glass Ionomer Decreases Lesion Progression and Bacterial Survival in an Experimental Model.

Purpose: To determine if sealing dentin caries with a resin-modified glass ionomer (RMGI) decreases the number of viable microorganisms and demineralization depth, using an experimental dentin caries model of biofilms of Streptococcus mutans (S. mutans).

Materials And Methods: Dentin caries lesions were created over a 15-day period on bovine dentin slabs with biofilms of S.

Correlation of Streptococcus mutans and Streptococcus sanguinis colonization and ex vivo hydrogen peroxide production in carious lesion-free and high caries adults.

Objective: This study was conducted to estimate oral colonization by Streptococcus mutans and Streptococcus sanguinis in adults with high and without any caries experience. Furthermore, differences in the amount of hydrogen peroxide (H2O2) produced by S. sanguinis isolated from both groups were assessed.

Fatty acid effect on sucrose-induced enamel demineralization and cariogenicity of an experimental biofilm-caries model.

Based on scarce evidence, fatty acids have been described as anticariogenic. The aim was to evaluate the effect of different types of fatty acids on enamel demineralization and on the cariogenic properties of Streptococcus mutans biofilms on a biofilm/caries model. Mature biofilms of S.

Cariogenicity of different commercially available bovine milk types in a biofilm caries model.

Purpose: This study's purpose was to assess the cariogenicity of commercial bovine milk types in an experimental biofilm/caries model.

Methods: Enamel and dentin slabs were used to grow biofilms of Streptococcus mutans UA159. Slabs/biofilms were exposed three times per day to commercial skim, semi-skim, whole, whole lactose-free, and whole with 10 percent sucrose-added bovine milk and to 10 percent sucrose and 0.

Cariogenic potential of commercial sweeteners in an experimental biofilm caries model on enamel.

Objective: Scarce evidence is available on the cariogenic potential of the widely used commercial sweeteners. The aim of this study was to evaluate the effect of several sweeteners on enamel demineralisation and on the cariogenic properties of Streptococcus mutans biofilms in an artificial caries model.

Methods: S.

Effect of bovine milk on Streptococcus mutans biofilm cariogenic properties and enamel and dentin demineralization.

Purpose: The purpose of this study was to evaluate the effect of whole bovine milk on Streptococcus mutans biofilms and on demineralization of enamel and root dentin.

Methods: S mutans UA159 biofilms were cultured on enamel and dentin bovine slabs of known surface hardness (SH) and exposed (8x/day) to 10% sucrose (positive control), whole bovine milk, and 4.5% lactose.

Fluoride content in toothpastes commercialized for children in Chile and discussion on professional recommendations of use.

Background: In Chile, no information is available regarding the soluble fluoride (F) content in the toothpastes commercialized for children and the country's guidelines recommend the use of F in toothpastes in an age-dependent concentration. No global consensus has been reached on this subject.

Aim: To determine the soluble F concentration in dentifrices for children sold in Chile and to discuss Chilean guidelines and professional recommendations of use.

Total and soluble fluoride content in commercial dentifrices in Chile.

Objective: Regardless of the total amount of fluoride (F) in a dentifrice, only the soluble fraction presents anti-caries activity and its concentration must be above 1000 ppm. No information regarding the quality of F in Chilean toothpastes is available. The aim of this study, therefore, was to determine the F concentration present in commercially-available toothpastes in Chile.