Publications by authors named "Cecilia Bergqvist"
The image analysis tool FRIC (Fluorescence Ratiometric Imaging of Chromatin) quantitatively monitors dynamic spatiotemporal distribution of euchromatin and total chromatin in live cells. A vector (pTandemH) assures stoichiometrically constant expression of the histone variants Histone 3.3 and Histone 2B, fused to EGFP and mCherry, respectively.
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- In most cells, heterochromatin (inactive) is found at the nuclear edge while euchromatin (active) is in the center, with variations in distribution during cell changes and conditions.
- A novel tool called Fluorescence Ratiometric Imaging of Chromatin (FRIC) was developed to monitor chromatin distribution dynamics in live cells qualitatively and quantitatively.
- FRIC demonstrated that heterochromatin clusters at the nuclear periphery and that its distribution is influenced by histone acetylation and the protein Samp1, highlighting its role in chromatin organization.
Samp1, spindle associated membrane protein 1, is a type II integral membrane protein localized in the inner nuclear membrane. Recent studies have shown that the inner nuclear membrane protein, Emerin and the small monomeric GTPase, Ran are direct binding partners of Samp1. Here we addressed the question whether Ran could regulate the interaction between Samp1 and Emerin in the inner nuclear membrane.
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- iPSCs (induced pluripotent stem cells) are promising for cell replacement therapies due to their ability to become any cell type, but their differentiation is often slow and unpredictable.
- The study investigates the role of Samp1 (Spindle Associated Membrane Protein 1), an INM protein that interacts with Lamin A/C, in the differentiation process of iPSCs.
- The findings suggest that higher levels of Samp1 can accelerate iPSC differentiation, with some cells showing early signs of becoming neurons within just six days, indicating its potential as a tool to enhance differentiation.
Here we describe multiplex suspension bead array systems that allow fast and reliable detection of reverse transcriptase (RT) PCR amplified filovirus genomes and also enable subtyping of Ebola virus species and Marburg virus strains. These systems have an analytical sensitivity equivalent to that of RT-PCR.
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