B-domain deleted factor VIII is aggregated and degraded through proteasomal and lysosomal pathways.

Factor VIII (FVIII) processing within mammalian cells is demonstrated to be much less efficient than proteins of similar size. The deletion of the B-domain from FVIII improves the level of production, due partly to the increase in mRNA synthesis. We aimed to characterise the cellular fate and the intracellular processing of the FVIII molecule devoid of B-domain.

Expression of the anti-apoptotic protein Hsp27 during both the keratinocyte differentiation and dedifferentiation of HaCat cells: expression linked to changes in intracellular protein organization?

We show here that Hsp27 increases its level of expression during the late phase of the keratinocyte differentiation of human HaCat cells. A similar phenomenon was observed when differentiated HaCat cells underwent a dedifferentiation process. In both cases, Hsp27 accumulated in the form of large native structures, which represent the chaperone active form of the protein.