The factors underlying the sudden infant death syndrome (SIDS) are still unknown, but in recent years much attention has been focused on the central cardiorespiratory control system. In the present work we analyzed the nucleus tractus solitarii (nTS) of 23 SIDS victims and 17 age-matched control cases. We studied the functional and morphological alterations of neurons and glial cells to evaluate the results of possible hypoxic-ischemic injury that could have led to sudden death.
View Article and Find Full Text PDFNine autistic children and 37 ethnically homogenous controls were enrolled in the study to assess their human leukocyte antigen (HLA) pattern, and eight healthy children were studied to define their peripheral blood cell subsets. We observed a significant decrease in CD4+ naive and an increase in CD4+ memory T cells in autistic children. These differences were significantly more pronounced in the autistic children bearing the HLA A2 and DR11 alleles.
View Article and Find Full Text PDFAim of this study is to determine the expression of apoptosis and Proliferating Cell Nuclear Antigen (PCNA) in the cardiac conduction system in crib death and explained death (ED) cases. Postnatal morphogenesis of the conducting tissue is an important part of its normal development. In the atrio-ventricular node (AVN) and His bundle (HB) it consists of degeneration, cell death and replacing in an orderly programmed way.
View Article and Find Full Text PDFBackground: In order to clarify the variable behaviour of transitional cell bladder carcinomas (TCBC) with same clinico-pathologic pattern, we investigated the prognostic significance of various biomarkers (PCNA, DNA, apoptosis, karyotype).
Materials And Methods: We studied 177 superficial TCBC (stage T1) undergoing transurethral resection (TUR). Analysis of biological indicators was performed on serial paraffin sections: DNA by static cytometry, karyotype by fluorescence in situ hybridisation (FISH), PCNA and apoptosis by immunohistochemistry.
Alterations of chromosomes 7 and 11 have been involved in the progression of atherosclerosis. Twenty-three carotid endarterectomy specimens were studied for the presence of alterations in chromosomes 7 and 11, and fibroblastic growth factor-3 (FGF-3) gene amplification. Besides classic histological stainings, immunophenotyping of cellular and vascular components and fluorescence in situ hybridization (FISH) were performed.
View Article and Find Full Text PDFChlamydia pneumoniae (CP), chromosomal alterations and apoptosis were suggested as contributing factors in the pathogenesis of atherosclerosis. Early (EP) and unstable plaques (UP) were studied in order to assess infiltrate composition, the apoptotic index, chromosome 7 stability and to investigate the concurrent presence of CP in EP and UP. Paraffin embedded sections of three iliac arteries and four aortas from young donors (EP), and four coronaries and nine carotid arteries (UP) were used.
View Article and Find Full Text PDFOur knowledge about the etiopathogenesis of nasal polyps (Nps) is still limited. In this study, in order to define the biological features of these neoformations, we investigated with immunohistochemistry the p53 over-expression and the proliferating cell nuclear antigen (PCNA) in 32 cases of Nps and in normal mucosa of 11 control cases. The evaluation of PCNA showed a wide range of indices (0.
View Article and Find Full Text PDFWe assessed the clinical and biological effects of high-dose, long-term Naltrexone (NTX) treatment in 11 children (3-11 years), who had been diagnosed as autistic. The drug was given following an open design, for 12 weeks. Beta-Endorphin (beta-END) was assayed in peripheral blood mononuclear cells after 1 and 3 months of treatment, and 6 months after the completion of the course.
View Article and Find Full Text PDFBackground: The different clinical evolution of gastric adenocarcinomas with the same clinico-pathologic characteristics prompted the authors to investigate the prognostic significance of different biological markers.
Methods: One hundred twenty-six preoperative cancer gastric biopsies, selected according the stage evaluated after following gastrectomy, were examined for DNA content by means the static cytometry, proliferating cell nuclear antigen (PCNA), p53 mutation, apoptosis by immunohistochemistry and karyotype using fluorescence in situ hybridization (FISH) techniques.
Results: The gastric cancers, ail belonging at stage III, were adenocarcinomas histologically typed as: 26 well differentiated (G1), 45 moderately differentiated (G2), 43 poorly differentiated (G3) and 12 of undifferentiated type with signet-ring cells.
The authors applied fluorescence in situ hybridization (FISH), using specific DNA probes for chromosome 7, to routine paraffin-embedded tissue sections obtained from 35 radical-prostatectomy specimens. Proliferative activity was also evaluated using static cytometry to assess DNA content and immunohistochemistry for proliferating cell nuclear antigen (PCNA) positivity. These results were correlated with each other and with the morphologic parameters.
View Article and Find Full Text PDFFluorescence in situ hybridization (FISH) using chromosome specific alpha-satellite DNA probes for chromosome 7, was performed on paraffin-embedded sections of 21 cases of human "unstable" atherosclerotic plaques, in order to determine whether trisomy 7, found by some Authors with conventional cytogenetics, is a biological characteristic of the atherosclerotic plaque. In 15 cases (71%) the FISH method showed smooth muscle cells with three or more spots. In particular the trisomy 7 was the most significant and constant result.
View Article and Find Full Text PDFAnn Ist Super Sanita
June 1994
Two conditions of temporary litter fostering, to water-drinking subjects and 28%-ethanol-drinking subjects, were compared to stay-with-mother condition and no-adult-present condition. Significant differences were found in the following variables: body weight during growth, self-feeding latency at weaning, timidity at one-month age, and, in adulthood, latency of females to contact an infant stimulus object and aggressive activities between males. It seems that the normal and the alcoholic virgin females had similar, long-lasting effect on emotionality of the fostered mice.
View Article and Find Full Text PDFElectroencephalogr Clin Neurophysiol
October 1988
The present paper reports a normative study of the BAEPs recorded from 80 normal full-term newborns by using a rarefaction click at 70, 60, 40, 20 dB HL. Positive, negative peaks and the relative amplitudes were measured. Means, standard deviations and 95% and 99% confidence limits were calculated for each component, for positive and negative IPLs and amplitude ratios.
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