A three-dimensional (3D) printing approach to fabricate an isolation chip for high throughput cultivation of environmental microbes.

The full plethora of environmental bacteria is often poorly represented as the majority remain difficult, if not impossible, to culture under standard laboratory settings. These bacteria often require native conditions for the formation of cell masses that collectively have higher chances of survival. With that, a 3D-printed version of the isolation chip (iChip) was used to cultivate bacteria from a tropical peat swamp prior to growth and maintenance .

sp. nov., isolated from subsurface soil and leaf litter in a tropical peat swamp forest.

A Gram-negative, filamentous aerobic bacterium designated as strain Mgbs1 was isolated on 12 April 2017 from the subsurface soil and leaf litter substrate at the base of a tree in a tropical peat swamp forest in the northern regions of the state of Selangor, Malaysia (3° 39' 04.7' N 101° 17' 43.7'' E).