Eighteen trinucleotide microsatellite loci for the solitary vespid wasp Monobia quadridens.

We identified 18 polymorphic, trinucleotide microsatellite loci for the solitary vespid wasp Monobia quadridens. These markers are to be used for parentage assessment and for studying population structure and inbreeding. Forty-eight diploid females from Southwest Michigan, USA were screened for allelic variation at each locus.

Expression of a beta-glucosidase gene results in increased accumulation of salicylic acid in transgenic Nicotiana tabacum cv. Xanthi-nc NN genotype.

A beta-glucosidase gene (bglA) from Butyrivibrio fibrisolvens H17c was cloned into the binary vector pGA482 under the control of the 35S Cauliflower Mosaic Virus (CaMV) promoter. A second construct was generated for accumulation of the bglA gene product in the vacuole of transformed tobacco plants. Reverse transcription-polymerase chain reaction analysis demonstrated that the bglA gene was expressed in 71% of cytosol-targeted and 67% of vacuole-targeted transgenic tobacco T(1) plants.