Comparable Skeletal and Dental Movements Achieved Using Conventional and Surgery-First Techniques in Class III Patients.

Purpose: The surgery-first (SF) approach to orthognathic surgery has been proposed as a more efficient method to correct the dentofacial deformity. This study aimed to evaluate if the magnitude of skeletal and dental changes achieved in Class III patients treated with either conventional orthognathic surgery (COS) or SF techniques differ.

Methods: A retrospective cohort study of Class III orthognathic surgery patients between January 2006 and May 2020 with available pre- and post-treatment lateral cephalograms was conducted at an academic institution.

Custom Made Device for Removal of Removable Partial Denture in a Patient with Manual Impairment.

Manual impairment could be part of the clinical presentation of a number of systemic conditions or traumas. Inability to successfully remove a removable partial denture is one of the contraindications of this type of treatment. This report presents a technique for fabrication of a custom device made chairside to assist a manually impaired patient due to osteoarthritis with the removal of a removable mandibular prosthesis.

A Firefly Luciferase Dual Color Bioluminescence Reporter Assay Using Two Substrates To Simultaneously Monitor Two Gene Expression Events.

Effective methods for monitoring eukaryotic gene expression and regulation based on bioluminescence - the emission of light by living organisms - are well established. Typically, the expression of a gene of interest is reported on with high sensitivity and over a wide dynamic range by the emission of light from a variety of engineered luciferase genes from beetles and marine organisms. The luciferase reporter genes are expressed downstream of the target gene or promoter and detected after exogenous addition of luciferin substrates.

Red-emitting chimeric firefly luciferase for in vivo imaging in low ATP cellular environments.

Beetle luciferases have been adapted for live cell imaging where bioluminescence is dependent on the cellular availability of ATP, O, and added luciferin. Previous Photinus pyralis red-emitting variants with high K values for ATP have performed disappointingly in live cells despite having much higher relative specific activities than enzymes like Click Beetle Red (CBR). We engineered a luciferase variant PLR3 having a K value for ATP similar to CBR and ∼2.