Method development for the determination of seven ginsenosides in three Panax ginseng reference materials via liquid chromatography with tandem mass spectrometry.

A new liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the analysis of ginsenosides in three Panax ginseng reference materials (RMs). Extraction procedures were optimized to recover neutral and malonyl-ginsenosides using a methanol-water extraction under basic conditions. Optimized mass fragmentation transitions were obtained for the development of a multiple reaction monitoring (MRM) detection method with electrospray ionization in negative and positive ion mode.

Development of Kudzu (Pueraria Montana var. lobata) Reference Materials for the Determination of Isoflavones and Toxic Elements.

Background: In collaboration with the Office of Dietary Supplements at the National Institutes of Health, the National Institute of Standards and Technology issued a suite of botanical matrix reference materials (RMs) and Standard Reference Material® (SRM) for determination of isoflavones and toxic elements in kudzu dietary supplement ingredients.

Objective: RM 8650 Pueraria montana var. lobata (Kudzu) Rhizome, SRM 3268 Pueraria montana var.

Determination of total arsenic and hydrophilic arsenic species in seafood.

Marine organisms are vital sources of staple and functional food but are also the major dietary route of human exposure to total arsenic. We surveyed the total arsenic content and the mass fractions of hydrophilic arsenic species from five different marine food types cutting across the food chain from microalgae, macroalgae, bivalve clam, crustaceans and finfish. Total arsenic was determined using inductively coupled plasma-mass spectrometry (ICP-MS) while arsenic speciation analysis was performed using high-performance liquid chromatography (HPLC) coupled to ICP-MS as the detector.

Determination of Curcuminoids in Turmeric Dietary Supplements by HPLC-DAD: Multi-laboratory Study Through the NIH-ODS/NIST Quality Assurance Program.

Background: Turmeric is a medicinal herb containing curcuminoids, used as quality markers in dietary supplements. In 2016, an AOAC First Action Official MethodSM was adopted for quantitation of curcuminoids and requires multi-laboratory reproducibility data for Final Action status.

Objective: To collect reproducibility data for the quantitation of curcuminoids in dietary supplements through the National Institutes of Health Office of Dietary Supplements/National Institute of Standards and Technology Quality Assurance Program (QAP).

Analytical Methodologies for the Determination of Organoarsenicals in Edible Marine Species: A Review.

Setting regulatory limits for arsenic in food is complicated, owing to the enormous diversity of arsenic metabolism in humans, lack of knowledge about the toxicity of these chemicals, and lack of accurate arsenic speciation data on foodstuffs. Identification and quantification of the toxic arsenic compounds are imperative to understanding the risk associated with exposure to arsenic from dietary intake, which, in turn, underscores the need for speciation analysis of the food. Arsenic speciation in seafood is challenging, owing to its existence in myriads of chemical forms and oxidation states.

Organoarsenicals in Seafood: Occurrence, Dietary Exposure, Toxicity, and Risk Assessment Considerations - A Review.

Diet, especially seafood, is the main source of arsenic exposure for humans. The total arsenic content of a diet offers inadequate information for assessment of the toxicological consequences of arsenic intake, which has impeded progress in the establishment of regulatory limits for arsenic in food. Toxicity assessments are mainly based on inorganic arsenic, a well-characterized carcinogen, and arsenobetaine, the main organoarsenical in seafood.

Characterization of triacontyl (C-30) liquid chromatographic columns.

Differences in the characteristics of seventeen commercial C-30 liquid chromatographic columns were studied for the separation of carotenoid isomers. A mixture consisting of nine xanthophyll and hydrocarbon carotenoids were separated under conditions carefully chosen to reveal changes in selectivity. The influence of the mobile phase composition, column temperature, and mobile phase flow rate were evaluated.

Challenges in Developing Analytically Validated Laboratory-Derived Dietary Supplement Databases.

The Dietary Supplement Label Database (DSLD) is sponsored by the Office of Dietary Supplements (ODS) and the National Library of Medicine (NLM). It provides a searchable, free database of the contents of ∼65,000 supplement labels. A companion database of analytically verified product labels [the Dietary Supplement Ingredient Database (DSID)] was created by ODS, NLM, and the USDA.