Potency against enterotoxemia of a recombinant Clostridium perfringens type D epsilon toxoid in ruminants.

Enterotoxemia, a disease that affects domestic ruminants, is caused mainly by the epsilon toxin from Clostridium perfringens type D. Its eradication is virtually impossible, control and prophylaxis are based on systematic vaccination of herds with epsilon toxoids that are efficient in inducing protective antibody production. The use of recombinant toxins is one of the most promising of these strategies.

[Viability of Tritrichomonas foetus after freezing with diferents cryoprotectors].

The aim of this work was to evaluate the viabilitity of Tritrichomonas foetus cryopreservated with glycerol and dimethilsulphosyde (DMSO) at -196 degrees C. Samples of the protozoa were thaw two days and 6, 12, 18, and 26 months after freezing to be evaluated. In the time analyzed, the freezing was viable in 60% and 90% of freeze samples with glycerol at 10% and DMSO at 12%, respectively.