Attachment of Respiratory Pathogens and to Denture Base Materials-A Pilot Study.

Denture prostheses are an ideal and extensive reservoir for microorganisms to attach to their surfaces. The aim of the study was to elucidate interactions between materials for the fabrication of denture bases and the attachment of microorganisms, focusing on respiratory pathogens and species. Specimens (6 mm × 1 mm) with a standardized surface roughness (Sa = 0.

Cross-Contamination Risk of Dental Tray Adhesives: An In Vitro Study.

Background: The aim of this study was to investigate the risk of cross-contamination in dental tray adhesives with reusable brush systems.

Methods: Four dental tray adhesives with different disinfectant components were examined for risk as a potential transmission medium for , , , , and . Bacterial and fungal strains were mixed with artificial saliva.

Dental tray adhesives and their role as potential transmission medium for microorganisms.

Objectives: This study aimed to evaluate the possible ability of dental impression tray adhesives to serve as a transmission medium for bacteria and fungi when reusable adhesive applicators are utilized.

Materials And Methods: Ten flasks with tray adhesive were monitored over a period of 12 weeks during clinical use for contamination with bacteria or fungi. Adhesive fluid samples were cultivated on eight different culture media.

A Rare Case of Wear Induced Complications after Cervical Disc Replacement.

Wear-induced complications after cervical disc replacement (CDR) are rare. A literature review on this topic found only a few case reports. We present a case of late complications after implantation of two cervical disc replacements, which resulted in infectious/abrasion-induced mixed-type inflammation with extensive osteolysis.

Discrimination of Human Pathogen Clostridium Species Especially of the Heterogeneous C. sporogenes and C. botulinum by MALDI-TOF Mass Spectrometry.

Clostridium species cause several local and systemic diseases. Conventional identification of these microorganisms is in part laborious, not always reliable, time consuming or does not always distinguish different species, i.e.

Influence of oral bacteria on adhesion of Streptococcus mutans and Streptococcus sanguinis to dental materials.

In this study, the effect of bacterial multispecies communities on the adhesion of and to dental restorative material was investigated. The saliva-coated specimens of zirconia and composite were incubated with the following combinations: single species, or ; two species, single species combined with other oral streptococci; multiple species, combination of , , and ssp.; and the two-species combinations.

Bacterial Colonization and Tissue Compatibility of Denture Base Resins.

Currently, there is minimal clinical data regarding biofilm composition on the surface of denture bases and the clinical tissue compatibility. Therefore, the aim of this experimental study was to compare the bacterial colonization and the tissue compatibility of a hypoallergenic polyamide with a frequently used PMMA resin tested intraorally in a randomized split-mouth design. Test specimens made of polyamide ( = 10) and PMMA ( = 10) were attached over a molar band appliance in oral cavity of 10 subjects.

The intra- and extraluminal appendiceal microbiome in pediatric patients: A comparative study.

Intestinal microbiota is involved in metabolic processes and the pathophysiology of various gastrointestinal disorders. We aimed to characterize the microbiome of the appendix in acute pediatric appendicitis comparing extraluminal and intraluminal samples.Between January and June 2015, 29 children (3-17 years, mean age 10.

The oral microbiome-the relevant reservoir for acute pediatric appendicitis?

Purpose: The oral microbiome has been related to numerous extra oral diseases. Recent studies detected a high abundance of oral bacteria in inflamed appendices in pediatric patients. To elucidate the role of oral bacteria in acute pediatric appendicitis, we studied the oral and appendiceal microbiome of affected children compared to healthy controls.

Identification of viridans streptococci With Matrix-Assisted Laser Desorption & Ionization Time-of-flight Mass Spectrometry by an In-house Method and a Commercially Available System.

Two matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS)-based methods were compared for their ability to identify viridans streptococci. One approach employed a reference database and software developed in-house. All inhouse measurements were performed using an Autoflex II Instrument (Bruker Daltonics GmbH, Germany).

Rapid identification of oral Actinomyces species cultivated from subgingival biofilm by MALDI-TOF-MS.

Background: Actinomyces are a common part of the residential flora of the human intestinal tract, genitourinary system and skin. Isolation and identification of Actinomyces by conventional methods is often difficult and time consuming. In recent years, matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) has become a rapid and simple method to identify bacteria.

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry.

Discrimination of Enterobacteriaceae and Non-fermenting Gram Negative Bacilli by MALDI-TOF Mass Spectrometry Matrix assisted laser desorption/ionization time of flight mass spectrometry (MALDI-TOF MS) has proven to be an effective identification tool in medical microbiology. Discrimination to subspecies or serovar level has been found to be challenging using commercially available identification software. By forming our own reference database and using alternative analysis methods, we could reliably identify all implemented Enterobacteriaceae and non-fermenting gram negative bacilli by MALDI-TOF MS and even succeeded to distinguish Shigella sonnei from Escherichia coli (E.

In vitro activities of levofloxacin, gatifloxacin, moxifloxacin and garenoxacin against Bacteroides fragilis strains evaluated by kill kinetics.

This study was designed to investigate the killing activity of levofloxacin, gatifloxacin, moxifloxacin and garenoxacin against 12 Bacteroides fragilis strains by kill kinetics over time. MIC values were determined by Etest and by agar dilution. B.

A step towards the discrimination of beta-lactamase-producing clinical isolates of Enterobacteriaceae and Pseudomonas aeruginosa by MALDI-TOF mass spectrometry.

Background: Matrix-Assisted Laser-Desorption/Ionization Time of Flight Mass Spectrometry (MALDI-TOF MS) has already proven to be a powerful tool for species identification in microbiological laboratories. As adequate and rapid screening methods for antibiotic resistance are crucially needed, the present study investigated the discrimination potential of MALDI-TOF MS among extended-spectrum-beta-lactamase (ESBL) or metallo-beta-lactamases- (MBL) producing and the nonproducing strains of Escherichia coli (n=19), Klebsiella pneumoniae (n=19), and Pseudomonas aeruginosa (n=38), respectively.

Material/methods: We used a MALDI-TOF MS protocol, usually applied for species identification, in order to integrate a screening method for beta-lactamases into the routine species identification workflow.

Microbial profile of patients with periodontitis compared with healthy subjects.

Objective: To define and compare the microbiologic profile of subgingival plaque in German patients with periodontitis (including aggressive and advanced chronic periodontitis) and healthy subjects and to determine significant association between isolates and clinical status. Aggregatibacter actinomycetemcomitans, Porphyromonas gingivalis, and Tannerella forsythia are major periodontal pathogens, though it is recognized that other species may also contribute to the pathogenesis of periodontal disease.

Method And Materials: Thirty-three patients with clinical and radiologic proof of aggressive and advanced chronic periodontitis and 20 healthy subjects were enrolled in this study.

[Microbiological aspects of subgingival plaque in patients with restorative treatments].

Objective: Our goal was to compare the microbial composition of subgingival plaque attached to different types of restorative materials.

Methods: The study included 19 patients. Samples of subgingival plaque were collected using sterile paper points.

[The impact of a sore throat score on clinical management of streptococcal angina].

Objective: To establish the validity and the role of a clinical sore throat score in clinical management of streptococcal angina on a first visit to a primary care provider.

Methods: This study included 168 patients. The sore throat score had 5 criteria: age, fever, absence of cough, tender anterior cervical nodes, tonsillar swelling or exudate.

[The distribution of anaerobic morpho-types in sub gingival plaque associated with periodontal disease].

The authors' goal has been to establish the distribution of anaerobic morpho-types in sub gingival plaque collected from patients with periodontal disease. The study included 26 patients. Samples of sub gingival plaque were transported in thioglycolate broth.