Culture medium and protein supplementation affect sensitivity of the mouse embryo assay in detecting Triton X-100.

Research Question: To what extent does the type and concentration of protein and the type of culture medium affect the sensitivity of the mouse embryo assay (MEA) to detect Triton X-100 (TX-100) in culture media?

Design: The effect of the concentration of bovine serum albumin (BSA) and human serum albumin (HSA) was assessed by supplementing media with 0.5 or 5 mg/ml. Potassium-supplemented simplex optimized medium (KSOM) and human tubal fluid (HTF) were used as complex and simple formulation media, respectively.

A diverse proteome is present and enzymatically active in metabolite extracts.

Metabolite extraction is the critical first-step in metabolomics experiments, where it is generally regarded to inactivate and remove proteins. Here, arising from efforts to improve extraction conditions for polar metabolomics, we discover a proteomic landscape of over 1000 proteins within metabolite extracts. This is a ubiquitous feature across several common extraction and sample types.

["The Nidcap gave me the impression of having a fairer and more objective perception of the child"].

Certified Neonatal Individualized Developmental Care and Assessment Program (Nidcap) childcare worker, Fabienne Grillère first experimented with this practice in the neonatology department before doing so in maternal and child care. Indeed, the principle of Nidcap care can be applied in different professional sectors since they refer to the premature or sick newborn and its observation. Its use brings an undeniable advantage in the support offered to the child and his family.

Clinical Management of Non-alcoholic Steatohepatitis and the Role of the Cardiologist.

Non-alcoholic fatty liver disease (NAFLD) is a spectrum of fat-associated liver conditions that increase risk of cardiovascular disease and mortality. The diagnosis and clinical management of NAFLD remain a challenge for cardiologists. Our group performed a systematic review in PubMed of the relationship between NAFLD and cardiovascular disease, identifying 35 relevant articles.

Effect of mouthwashes on the force decay of polymeric ligature chains used for dental purposes: a systematic review and meta-analysis.

Background: External factors such as the daily use of antimicrobial mouthwashes to maintain oral hygiene and to reduce the microbial activity can contribute to alter the mechanical properties of the elastomeric chains used during orthodontic treatments, causing loss of effectiveness. This systematic review and a meta-analysis assessed the rate of force decay and degradation of the polymeric chains depending on the type of mouthwash.

Methods: A systematic search of the literature were there was an exposure of orthodontic elastomeric chains to certain mouthwashes was conducted in the electronic databases of PubMed, Cochrane Library (CENTRAL), Scopus, EMBASE and Web of Science, as well as grey literature (Opengrey).

Fertility specialists' views, behavior, and attitudes towards the use of endometrial scratching in Italy.

Background: Endometrial scratching (ES) or injury is intentional damage to the endometrium performed to improve reproductive outcomes for infertile women desiring pregnancy. Moreover, recent systematic reviews with meta-analyses and randomized controlled trials demonstrated that ES is not effective, data on the safety are limited, and it should not be recommended in clinical practice. The aim of the current study was to assess the view and behavior towards ES among fertility specialists throughout infertility centers in Italy, and the relationship between these views and the attitudes towards the use of ES as an add-on in their commercial setting.

First babies conceived with Automated Intracytoplasmic Sperm Injection.

Research Question: Can an automated sperm injection robot perform Automated Intracytoplasmic Sperm Injection (ICSIA) for use in human IVF?

Design: The ICSIA robot automated the sperm injection procedure, including injection pipette advancement, zona pellucida and oolemma penetration with piezo pulses, and pipette removal after sperm release. The robot was first tested in mouse, hamster and rabbit oocytes, and subsequently using discarded human oocytes injected with microbeads. A small clinical pilot trial was conducted with donor oocytes to study the feasibility of the robot in a clinical setting.

[« I believe that children should know how they were conceived »].

The decision to become a donor is an altruistic one. It allows infertile couples wishing to have a child to realize their dream. Although there have been developments in recent years concerning the lifting of donor anonymity, there is still progress to be made.

[« I was moved by an absolute urgency: I had to know what had always been kept from me »].

In certain family configurations, the secrecy of origins is fiercely maintained. The revelation can then occur suddenly, causing a radical upheaval for the person concerned. This is what happened to Alexandre Mercier, who agreed to share his experience with us.

[« The desire to know where I came from pushed me to start the research »].

This interview retraces the journey of a man who, wishing to know where he came from, decided to go in search of his origins. From wanderings to hesitations, from bitterness to determination, Arthur Kermalvezen Fournis describes the different steps that led him to the truth. A painful but salutary fight.

Features of Obstructive Sleep Apnea in Children with and without Comorbidities.

Background: To compare the clinical and polysomnographic features of obstructive sleep apnea (OSA) in children with adenotonsillar hypertrophy (Group A) and comorbidities (Group B).

Methods: A five-year prospective study using nocturnal polysomnography before and after treatment.

Results: We included 168 patients: 121 in Group A and 47 in Group B, with differences in age (6.

["The association proposes animations and moments of distraction to the hospitalized children"].

Today, Le Rire médecin has thirty years of experience. It has 120 trained professional clowns who make more than 85,000 individualized visits to 58 pediatric wards in France. Caroline Simonds, the association's founding clown, and Dominique Valteau-Couanet, a pediatrician and former head of department at the Gustave-Roussy Institute in Villejuif (94), met for a two-voice, two-hearted discussion.

["The association proposes animations and moments of distraction to the hospitalized children"].

The association Les Blouses roses intervenes in many hospitals in France, especially at the bedside of hospitalized children. It tries to bring them various animations, fantasy and gaiety in the dark universe of the disease. This is not always an easy task, but it is supported by the will to accompany the young patient and his family against all odds.

Local tumour nanoparticle thermal therapy: A promising immunomodulatory treatment for canine cancer.

Distinct thermal therapies have been used for cancer therapy. For hyperthermia (HT) treatment the tumour tissue is heated to temperatures between 39 and 45°C, while during ablation (AB) temperatures above 50°C are achieved. HT is commonly used in combination with different treatment modalities, such as radiotherapy and chemotherapy, for better clinical outcomes.

["The parent has become a true partner thanks to developmental care"].

Malika Jourdain has been a nursery nurse for nearly thirty years and works in neonatology at the children's hospital of La Tronche, in the Isère region. Her rich experience has enabled her to take part in some of the developments that have led to a better understanding of the role of parents with their premature newborn. In particular, the introduction of developmental care has led to upheavals in neonatal services.

Characterization and comparison of commercial oils used for human embryo culture.

Study Question: Are there significant differences between the available commercial oil brands used for human IVF?

Summary Answer: Important differences have been detected among the tested oil brands in their potential to stabilize culture conditions and, more importantly, in their direct effect on embryo development and viability.

What Is Known Already: Mineral oil is a critical component of the human culture system due to its protective and stabilizing roles during in vitro embryo development. Many different oils are available on the market, with differences in their viscosity, density and overall quality.