Publications by authors named "Cassandra R Woolley"
Article Synopsis
- Current short-read AIRR-seq methods have limitations in resolving the constant (C) region of antibody transcripts, prompting the development of a new approach called FLAIRR-seq, which achieves 99.99% accuracy in generating human antibody heavy chain transcripts.
- FLAIRR-seq was validated against standard methods, showing comparable results while revealing previously undocumented heavy chain gene features and enabling detailed characterization of antibody gene diversity.
- This new method identified 32 unique IGHC alleles in ten individuals, with 87% being uncharacterized, thus providing the most comprehensive analysis of antibody repertoires to date.
Article Synopsis
- Long-read sequencing technologies like isoform sequencing can accurately capture full-length mRNA transcript isoforms, which are essential for studying lymphocyte functional plasticity in health and disease.
- Despite their importance, comprehensive long-read transcriptomes for human circulating lymphocytes have been lacking.
- In this study, researchers isolated four lymphocyte populations from a healthy male donor, identified many novel transcript isoforms, and made their high-quality data publicly available to aid future transcriptomic research.
The Fc receptor for IgM, FcMR, is unusual in that it is preferentially expressed by cells of the adaptive immune system. It is, moreover, the only constitutively expressed Fc receptor on human T cells. Efforts to decipher the normal functions of FcMR have been complicated by species-specific expression patterns in lymphocytes from mice (B cells) versus humans (B, NK, and T cells).
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