Publications by authors named "Cassandra N Fuller"
The "Histone Code" is comprised of specific types and positions of post-translational modifications (PTMs) which produce biological signals for gene regulation and have potential as biomarkers for medical diagnostics. Previous work has shown that electron-based fragmentation improves the sequence coverage and confidence of labile PTM position assignment. Here, we evaluated two derivatization methods (e.
View Article and Find Full Text PDFThe amino acid position within a histone sequence and the chemical nature of post-translational modifications (PTMs) are essential for elucidating the "Histone Code". Previous work has shown that PTMs induce specific biological responses and are good candidates as biomarkers for diagnostics. Here, we evaluate the analytical advantages of trapped ion mobility (TIMS) with parallel accumulation-serial fragmentation (PASEF) and tandem mass spectrometry (MS/MS) for bottom-up proteomics of model cancer cells.
View Article and Find Full Text PDFArticle Synopsis
- - Histone proteins, crucial for gene regulation in eukaryotes, are modified post-translationally (PTMs), and understanding these modifications can help link them to biological processes like DNA transcription, replication, and repair.
- - The study details a new high-throughput method using advanced techniques like liquid chromatography and mass spectrometry to analyze histone PTMs from biological samples effectively.
- - The method employs innovative data acquisition strategies that help accurately identify and assign the various biologically important histone modifications based on specific characteristics during analysis.