Publications by authors named "Casey L Ruark-Seward"
Tomato spotted wilt virus (TSWV) is a generalist pathogen with one of the broadest known host ranges among RNA viruses. To understand how TSWV adapts to different hosts, we experimentally passaged viral populations between two alternate hosts, Emilia sochifolia and Datura stramonium, and an obligate vector in which it also replicates, western flower thrips (Frankliniella occidentalis). Deep sequencing viral populations at multiple time points allowed us to track the evolutionary dynamics of viral populations within and between hosts.
View Article and Find Full Text PDFNematode-infecting RNA viruses have recently been discovered via transcriptome sequencing. In soybean cyst nematode (SCN; Heterodera glycines), seven single-stranded RNA viruses have been identified from transcriptome data and experimentally confirmed with qRT-PCR and Sanger sequencing. Presently, there is still much unknown about the relationship between these viruses and the nematode host.
View Article and Find Full Text PDFArticle Synopsis
- A new fluorescence hybridization (FISH) protocol was created for nematodes that uses electroporation to introduce nucleic acid probes directly into the organism.
- This streamlined method reduces chemical wash steps, leading to lower specimen loss and faster protocol times, specifically optimized for juveniles of soybean cyst nematodes.
- The protocol allows for the simultaneous detection of two different targets using long wavelength fluorophores, which enhances hybridization sensitivity and may be adapted for other nematode species and life stages.