Effect of nutritional stress and serum starvation on the optical absorbance of normal and malignant epithelial cell lines.

This brief report aimed to investigate the optical absorbance spectra of normal, dysplastic, and malignant epithelial cell lines under normal and nutritional stress conditions. HaCAT (keratinocyte), DOK (oral dysplastic), and oral squamous cell carcinoma (OSCC) cell lines (CA1, Luc4, SCC9) were evaluated regarding their optical absorbance after culture with 0-10% fetal bovine serum. Absorbance measurements indicated that HaCAT under serum starvation exhibited higher absorbance at blue (430 nm) and near-infrared (906 nm) wavelengths.

Neurobiology of L-proline: From molecules to behavior.

L-proline is an amino acid with a unique cyclic structure, involvement in various physiological processes, such as protein synthesis, collagen production, and neurotransmission. This review explores the complex roles of proline in the central nervous system (CNS), where it contributes to both excitatory and inhibitory neurotransmission. Additionally, L-proline has distinct metabolic functions attributed to its structural properties.

First report of a cat (Felis catus) infected by Leishmania (Viannia) guyanensis from Southeastern Brazil.

Feline leishmaniasis is increasingly recognized as a disease affecting cats worldwide, with notable prevalence in regions surrounding the Mediterranean Basin and in Brazil. An approximately one-year-old domestic shorthair cat was presented with small papules and nodules (2 mm) on the nasal planum. Physical examination and routine blood analyses were normal.

Root Development of Tomato Plants Infected by the Cacao Pathogen Moniliophthora perniciosa Is Affected by Limited Sugar Availability.

Moniliophthora perniciosa is the causal agent of the witches' broom disease of cacao (Theobroma cacao), and it can infect the tomato (Solanum lycopersicum) 'Micro-Tom' (MT) cultivar. Typical symptoms of infection are stem swelling and axillary shoot outgrowth, whereas reduction in root biomass is another side effect. Using infected MT, we investigated whether impaired root growth derives from hormonal imbalance or sink competition.

Cascade reservoirs affect mercury concentrations in fish from Teles Pires river, Brazilian Amazon.

Hydroelectric reservoirs favor mercury contamination in biota, but the contamination in cascade reservoirs is not yet clear. We investigated total mercury (THg) contamination in fish in four cascade reservoirs in the Brazilian Amazon between August 2022 and April 2023. Overall, downstream predatory fish showed higher mercury concentrations than those upstream.

The Patterns of P53, E-Cadherin, β-Catenin, CXCR4 and Podoplanin Expression in Oral Squamous Cell Carcinoma Suggests a Hybrid Invasion Model: an Immunohistochemical Study on Tissue Microarrays.

Purpose: Oral squamous cell carcinoma (OSCC) is a significant public health challenge associated with high mortality rates primarily due to its invasive and metastatic behavior. This study aimed to evaluate the expression patterns of five critical biomarkers: β-catenin, E-cadherin, podoplanin (PDPN), CXCR4, and p53 in OSCC tissues and to investigate their correlations with clinicopathologic features and patient outcomes.

Methods: We conducted an immunohistochemical analysis utilizing tissue microarrays (TMAs) from 95 patients diagnosed with primary OSCC.

Assessing neighbourhood-scale BTI spray applications and laboratory-based mortality testing on larval development.

Mosquitoes, particularly , pose significant public health risks by transmitting diseases like dengue, zika and chikungunya. var. (BTI) is a crucial larvicide targeting mosquitoes while sparing other organisms and the environment.

A Meal with Ultra-Processed Foods Leads to a Faster Rate of Intake and to a Lesser Decrease in the Capacity to Eat When Compared to a Similar, Matched Meal Without Ultra-Processed Foods.

: It is unknown whether the negative health effects associated with ultra-processed foods (UPFs) are due to their nutritional composition or to the extent of food processing itself. We evaluated the impact of a test meal composed only of UPF, according to the NOVA classification, compared to a similar meal without UPF in adults with obesity. : This is a parallel, randomized trial.

Vaccination: a look at the social representations of Brazilian children.

Introduction: The pandemic caused by COVID-19 has accentuated the debate on the need for vaccination and called into question the need to increasingly bring this topic, which is widely disseminated in the scientific world, to school classes at all schooling phases. In this scenario, science education plays a key role in disseminating knowledge about the importance of vaccination and the impacting factors of a lack of immunization. In order to better understand this movement, it is necessary to understand the representations of individuals as a way of broadening paths to change this scenario.

Advancing Health and Sustainability: A Holistic Approach to Food Production and Dietary Habits.

Producing healthier food requires expertise in methods that yield significant benefits for human health, sustainability, economic growth, cultural heritage, and overall well-being. Investing in conscientious and sustainable food systems can improve individual and planetary quality of life by preventing diseases, delaying ageing, and enhancing well-being. While healthy eating habits begin at home, schools play a pivotal role in reinforcing them from an early age.

TiO nanotubes incorporated into a glaze-coating ceramic: surface roughness, color, and antibiofilm activity.

This study evaluated the surface roughness, color change, and antibacterial effect of a ceramic glaze enhanced with TiO nanotubes (n-TiO). n-TiO (0, 2, 2.5, and 5 wt%) was added to a ceramic glaze powder, applied to the surface of forty feldspathic ceramic specimens, and sintered.

Sex-and Stress-Dependent Plasticity of a Corticotropin Releasing Hormone / GABA Projection from the Basolateral Amygdala to Nucleus Accumbens that Mediates Reward Behaviors.

Background: Motivated behaviors are executed by refined brain circuits. Early-life adversity (ELA) is a risk for human affective disorders involving dysregulated reward behaviors. In mice, ELA causes anhedonia-like behaviors in males and augmented reward motivation in females, indicating sex-dependent disruption of reward circuit operations.

Improved production of palitantin by design of experiments and semi-synthesis of palitantin derivatives for bioactivity assessment.

A new method for the production and isolation of (+)-palitantin (1) is herein reported, from cultures of the fungal strain Penicillium sp. AMF1a. (+)-Palitantin was isolated in 160 mg/L yield, as an alternative procedure to obtain 1 at a larger scale.

Repercussions of Long-Term Naproxen Administration on LPS-Induced Periodontitis in Male Mice.

Aims: Chronic periodontitis is the sixth most prevalent disease worldwide and the leading cause of tooth loss in adults. With growing attention on the role of inflammatory and immune responses in its pathogenesis, there is an urgent need to evaluate host-modulatory agents. Non-steroidal anti-inflammatory drugs (NSAIDs) drugs play a crucial role in managing inflammatory conditions.

Heparan sulfate in cerebrospinal fluid as a biomarker to assess disease severity and for treatment monitoring in patients with Mucopolysaccharidosis Type II: a position statement.

Patients with mucopolysaccharidosis type II (MPS II) can present with a severe neuronopathic phenotype or an attenuated non-neuronopathic phenotype. In the light of the recent development of drugs that cross the blood-brain barrier for treatment of neurologic MPS II symptoms, it is critical to define biomarkers that objectively differentiate phenotypes and monitor therapeutic outcomes of advanced treatments. In December 2023, a panel of Brazilian experts discussed the potential of quantifying heparan sulfate (HS) in the cerebrospinal fluid (CSF) as a biomarker for assessing neurological impairment in patients with MPS II, as well as the potential of the molecule as an objective parameter for therapeutic monitoring.

Low Agreement Among Guidelines for Primary Prevention Implantable Cardioverter-Defibrillator Recommendations in Hypertrophic Cardiomyopathy.

Sudden cardiac death (SCD) risk stratification and primary prevention implantable cardioverter-defibrillator (ICD) recommendations are based on differing strategies for hypertrophic cardiomyopathy (HCM). This study aimed to evaluate the impact of the 2023 European Society of Cardiology (ESC) guidelines on the 2014 ESC and the 2024 American Heart Association (AHA)/American College of Cardiology (ACC) systems in terms of primary prevention ICD recommendations for HCM. A cohort of 200 patients with HCM in Brazil was assessed for SCD risk profile according to current guidelines.

Impact of Individuals' Biological and Meals' Nutritional Characteristics on the Thermic Effect of Food in Humans: Meta-Regression of Clinical Trials.

Context: The thermic effect of food (TEF) may be a therapeutic target for the prevention and treatment of obesity.

Objective: The impact of different biological and nutritional characteristics on TEF in humans was analyzed.

Data Sources: The MEDLINE/PubMed, Embase, Cochrane Central Register of Controlled Trials, Web of Science, and Latin American and Caribbean Health Sciences Literature databases were searched until November 2023 without language restrictions.

Comprehensive analysis of the electronic, thermodynamic, and spectroscopic properties of a Cu(II)-based complex with 1,10-phenanthroline and L-glutamine.

This study reports additional information's of geometric, electronic, thermodynamic, and vibrational properties of a Cu(II) complex with 1,10-phenanthroline and L-glutamine ligands. The experimental spectroscopic analyses were corroborated by density functional theory (DFT). Furthermore, these properties were evaluated using an implicit solvation method with water and methanol solvents, including vacuum condition.

GlyT1 inhibition promotes neuroprotection in the middle cerebral artery occlusion model through the activation of GluN2A-containing NMDAR.

Glycine Transporter Type 1 (GlyT1) inhibition confers neuroprotection against different forms of cerebral damage. This effect occurs through the elevation of synaptic glycine concentrations, which enhances N-methyl-d-aspartate receptor (NMDAR) activation by glutamate. To investigate the neuroprotective mechanism of GlyT1 inhibition, we used the Middle Cerebral Artery Occlusion (MCAO) model in male C57BL/6 mice, aged 10-12 weeks.