Viability discrimination of bacterial microbiomes in home kitchen dish sponges using propidium monoazide treatment.

Dish sponges are known to support the proliferation of human bacterial pathogens, yet they are commonly used by consumers. Exposure to foodborne pathogens via sponge use may lead to illness, a serious concern among susceptible populations. The extent of exposure risks from sponge use has been limited by constraints associated with culture-independent or dependent methods for bacterial community characterization.

Evaluation of the kitchen microbiome and food safety behaviors of predominantly low-income families.

Bacterial pathogens in the domestic environment present a risk to residents, particularly among susceptible populations. However, the impact of consumer demographic characteristics and food handling methods on kitchen microbiomes is not fully understood. The domestic kitchen bacterial communities of ten predominantly low-income families in Houston, TX, were assessed in conjunction with a cross-sectional food safety survey to evaluate differences in household and surface-specific microbiomes and bacterial foodborne pathogen presence.

Food Safety Attitudes, Behaviors, and Hygiene Measures among Predominantly Low-Income Parents in Houston, Texas.

Abstract: Foodborne infections in the United States affect racial-ethnic minority and low-income populations at higher rates than the general population. To identify the prevalence of food safety behaviors and demographic characteristics associated with food handling practices among a susceptible, high-risk population, a cross-sectional survey was administered to 106 parents with children enrolled at two elementary schools serving predominantly low-income families in Houston, Texas. Relationships between demographic characteristics and food safety behavioral outcomes were examined using cross-tabulations and Fisher's exact test.

Daratumumab infusion reaction rates pre- and post-addition of montelukast to pre-medications.

Daratumumab, a CD38-directed monoclonal antibody indicated for multiple myeloma treatment in adult patients, is associated with a high incidence of infusion-related reactions (IRRs). Due to CD38 receptor presence in the lungs, many reactions present similarly to asthma or allergic rhinitis. Montelukast, a leukotriene receptor antagonist, has been hypothesized to reduce daratumumab IRRs due to its efficacy in treating allergic rhinitis and asthma and the presence of leukotriene receptors in the lungs.

Isolated Pectoralis Minor Tendon Rupture with Subclavian Vein Thrombosis.

Isolated insertional ruptures of the pectoralis minor tendon at the coracoid process are a rare condition. Hitherto, very few cases have been reported in the literature. A precise diagnosis is often difficult to obtain and commonly requires advanced imaging to confirm the suspicion and rule out concomitant injuries.

Desire to Quit Smoking in an Outpatient Population of Persons with Serious Mental Illness.

National survey data indicates that about 32% of adults with any mental illness smoke, compared with 23% of adults without a psychiatric disorder. Smoking rates are higher in clinical populations, where up to 53% of persons with serious mental illnesses (schizophrenia and bipolar disorder) are estimated to smoke. Despite higher rates of smoking among persons with mental illnesses, motivation to quit in this population is similar to that of the general population of smokers.

Population Dynamics of Listeria monocytogenes, Escherichia coli O157:H7, and Native Microflora During Manufacture and Aging of Gouda Cheese Made with Unpasteurized Milk.

Abstract: Cheeses made with unpasteurized milk are a safety concern due to possible contamination with foodborne pathogens. Listeria monocytogenes and Escherichia coli O157:H7 have been implicated in several outbreaks and recalls linked to Gouda cheese made with unpasteurized milk. The U.

Multistate Outbreaks of Foodborne Illness in the United States Associated With Fresh Produce From 2010 to 2017.

In the United States, the consumption of fresh fruits and vegetables has increased during recent years as consumers seek to make healthier lifestyle choices. However, the number of outbreaks associated with fresh produce that involve cases in more than one state (multistate) has increased concomitantly. As the distance along the farm-to-fork continuum has lengthened over time, there are also more opportunities for fresh produce contamination with bacterial pathogens before it reaches the consumer.

Metagenomics of pasteurized and unpasteurized gouda cheese using targeted 16S rDNA sequencing.

Background: The microbiome of cheese is diverse, even within a variety. The metagenomics of cheese is dependent on a vast array of biotic and abiotic factors. Biotic factors include the population of microbiota and their resulting cellular metabolism.

Control of Listeria monocytogenes in Caramel Apples by Use of Sticks Pretreated with Potassium Sorbate.

A multistate listeriosis outbreak associated with caramel apples from 2014 to 2015 prompted research on the survival of Listeria monocytogenes in fresh apples and caramel apples. Research indicated that stem end-inoculated caramel apples with stick insertion allowed for the survival and growth of L. monocytogenes at both refrigeration and ambient temperatures.

The intrinsic vulnerability of networks to epidemics.

Contact networks are convenient models to investigate epidemics, with nodes and links representing potential hosts and infection pathways, respectively. The outcomes of outbreak simulations on networks are driven both by the underlying epidemic model, and by the networks' structural properties, so that the same pathogen can generate different epidemic dynamics on different networks. Here we ask whether there are general properties that make a contact network intrinsically vulnerable to epidemics (that is, regardless of specific epidemiological parameters).

A unifying framework for fast randomization of ecological networks with fixed (node) degrees.

The Curveball algorithm is an efficient and unbiased procedure for randomizing bipartite networks (or their matrix counterpart) while preserving node degrees. Here we introduce two extensions of the procedure, making it capable to randomize also unimode directed and undirected networks. We provide formal mathematical proofs that the two extensions, as the original Curveball, are fast and unbiased (i.

A Rapid Combinatorial Approach to Assembling Synthetic Prokaryotic and Eukaryotic Protein Expression Vectors.

Vector construction and gene cloning are ubiquitous techniques essential to all fields of biological and medical research. They are the first steps in many endeavors leading to expressing proteins to understand gene function and regulation. However, they can often be rate-limiting, particularly in multi-gene studies, due to the time and effort required to assemble gene constructs and to identify the optimal constructs for protein expression.

Construction of CRISPR Libraries for Functional Screening.

Identification of gene function has been aided by the ability to generate targeted gene knockouts or transcriptional repression using the CRISPR/CAS9 system. Using pooled libraries of guide RNA expression vectors that direct CAS9 to a specific genomic site allows identification of genes that are either enriched or depleted in response to a selection scheme, thus linking the affected gene to the chosen phenotype. The quality of the data generated by the screening is dependent on the quality of the guide RNA delivery library with regards to error rates and especially evenness of distribution of the guides.

Predictors of Labor Force Status in a Random Sample of Consumers with Serious Mental Illness.

Employment among persons with severe mental illness has been challenging. Supported employment programs have had some success; however, much remains to be understood about client motivations for employment. A labor force participation study was mailed to persons receiving services in a Midwestern state's publicly funded behavioral health system, and a random sample of participants resulted in 964 valid surveys.

Growth Kinetics in Milkshakes Made from Naturally and Artificially Contaminated Ice Cream.

This study assessed the growth of in milkshakes made using the process-contaminated ice cream associated with a listeriosis outbreak in comparison to milkshakes made with artificially contaminated ice cream. For all temperatures, growth kinetics including growth rates, lag phases, maximum populations, and population increases were determined for the naturally and artificially derived contaminants at 5, 10, 15, and 25°C storage for 144 h. The artificially inoculated presented lower growth rates and shorter lag phases than the naturally contaminated populations at all temperatures except for 5°C, where the reverse was observed.

Is Total Disk Replacement a Cost-effective Treatment for Cervical Degenerative Disk Disease?

Study Design: A retrospective study.

Objective: The objective of this study was to assess the cost-effectiveness and clinical outcome of motion-preserving versus fusion procedures in cervical spine surgery.

Summary Of Background Data: During the last decade there has been a huge growth in spine surgery with a concurrent increase in the economic burden.

Network analysis reveals why Xylella fastidiosa will persist in Europe.

The insect vector borne bacterium Xylella fastidiosa was first detected in olive trees in Southern Italy in 2013, and identified as the main culprit behind the 'olive quick decline syndrome'. Since then, the disease has spread rapidly through Italy's main olive oil producing region. The epidemiology of the outbreak is largely unstudied, with the list of X.

Erratum: Proof of uniform sampling of binary matrices with fixed row sums and column sums for the fast Curveball algorithm [Phys. Rev. E 91, 042812 (2015)].

This corrects the article DOI: 10.1103/PhysRevE.91.

Fate of Listeria monocytogenes in Fresh Apples and Caramel Apples.

An outbreak of listeriosis in late 2014 and early 2015 associated with caramel apples led to questions about how this product became a vector for Listeria monocytogenes. This investigation aimed to determine information about the survival and growth of L. monocytogenes in both fresh apples and caramel apples, specifically examining the effects of site and level of inoculation, inoculum drying conditions, and storage temperature.

[History of the German Spine Society].

The objective of this article is to summarize the history of the German Spine Society (DWG). This society resulted in the year 2006 after several attempts from the fusion of two established German societies, which were dealing with topics around the spine, der "German Society for Spine Research" founded in the year 1958 and the "German Society for Spine Surgery" founded in the year 1987. This fusion was the beginning of a success story, as from this time on the annual membership increased so much that the DWG became the largest spine society in Europe and one of all spine societies worldwide.

Proof of uniform sampling of binary matrices with fixed row sums and column sums for the fast Curveball algorithm.

Randomization of binary matrices has become one of the most important quantitative tools in modern computational biology. The equivalent problem of generating random directed networks with fixed degree sequences has also attracted a lot of attention. However, it is very challenging to generate truly unbiased random matrices with fixed row and column sums.

[Posterior lumbar interbody fusion implants. Software assisted planning--preliminary results].

Background: Sagittal imbalance, adjacent segment degeneration, and loss of correction due to cage sintering are the main reasons for revision surgery after lumbar fusion. Based on the experience from hip and knee replacement surgery, preoperative software-assisted planning combined with the corresponding cages is helpful to achieve better long-term results.

Objectives: Evaluation of the procedure regarding intraoperative application of preoperative planning and examination to what extent the planning was correct.

Impact of wine manufacturing practice on the occurrence of fining agents with allergenic potential.

Proteinogenic wine fining agents are hidden allergens and could present a risk for consumers with allergies. Therefore, the European Parliament adopted Directive 2003/89/EC amending Directive 2000/13/EC to declare ingredients, contaminations and processing aids that are known to trigger allergic reactions. The Amendment Regulation (EU) 1266/2010 excluded the labelling of wines which are processed with hen's egg and products thereof until 30 June 2012 to get more scientific findings.

Development of a sensitive ELISA for the detection of casein-containing fining agents in red and white wines.

Fining of wine with proteinogenic fining agents such as casein from cow's milk is a traditional and commonly used technique all over the world. Casein and other proteins from cow's milk are well-known food allergens, which pose a risk for allergic consumers. Temporary regulations exempting the labeling of milk and products thereof in wine expired.