Morphological, biochemical, and growth characteristics of pseudomonas cepacia from distilled water.

Studies were conducted on three strains of Pseudomonas cepacia isolated and maintained in distilled water and on a laboratory-subcultured strain transferred to distilled water. Optimum growth rates and maximum population yields of the four strains in distilled water were obtained at 37 C, although high population levels (10(6)-10(7)/ml) were reached and maintained over extended incubation periods at temperatures from 18 C to 42 C. Two strains were able to grow in distilled water at temperatures ranging from 12 C to 48 C and to survive 48 h and 21 days at 50 C and 10 C, respectively.

Factors affecting comparative resistance of naturally occurring and subcultured Pseudomonas aeruginosa to disinfectants.

A strain of Pseudomonas aeruginosa was isolated in pure culture from the reservoir of a hospital mist therapy unit by an extinction-dilution technique; its natural distilled water environment was used as a growth and maintenance medium. After a single subculture on Trypticase soy agar, the strain showed a marked decrease in resistance to inactivation by acetic acid, glutaraldehyde, chlorine dioxide, and a quaternary ammonium compound when compared with naturally occurring cells grown in mist therapy unit water. The following factors were observed to affect the relative resistances of naturally occurring and subcultured cells of the P.

Pseudomonas aeruginosa: growth in distilled water from hospitals.

Pseudomonas aeruginosa can grow relatively fast in distilled water obtained in hospitals and achieve high cell contaminations which remain stable for long periods of time. Cells grown in distilled water react quite differently to chemical and physical stresses than cells grown in standard laboratory culture media.