Atacama desert is a source of new Micromonospora strains: description of Micromonospora sicca sp. nov.

Several strains were isolated from subsurface soil of the Atacama Desert and were previously assigned to the Micromonospora genus. A polyphasic study was designed to determine the taxonomic affiliation of isolates 4G51, 4G53, and 4G57. All the strains showed chemotaxonomic properties in line with their classification in the genus Micromonospora, including meso-diaminopimelic acid in the cell wall peptidoglycan, MK-9(H) as major respiratory quinone, iso-C and iso-C as major fatty acids and diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol as major polar lipids.

The Association between Bronchiectasis and Chronic Obstructive Pulmonary Disease: Data from the European Bronchiectasis Registry (EMBARC).

COPD and bronchiectasis are commonly reported together. Studies report varying impacts of co-diagnosis on outcomes, which may be related to different definitions of disease used across studies. To investigate the prevalence of chronic obstructive pulmonary disease (COPD) associated with bronchiectasis and its relationship with clinical outcomes.

sp. nov. and sp. nov., isolated from the rhizosphere of a plant growing in the Salar de Tara region of the Central Andes in Chile.

Two novel strains, STR1-7 and STR1S-6, were isolated from the rhizosphere of a plant growing in the Salar de Tara region of the Atacama Desert, Chile. Chemotaxonomic, cultural and phenotypic features confirmed that the isolates belonged to the genus . They grew from 20 to 37 °C, from pH7 to 8 and in the presence of up to 3 %, w/v NaCl.

sp. nov. and sp. nov., two endophytic metal-resistant bacteria isolated from (Cav.) G. Don and (Humb. et Bonpl. ex Willd) M.C. Johnston.

Four Gram-positive, aerobic, catalase- and oxidase-negative, rod-shaped, motile endophytic bacterial strains, designated NM3R9, NE1TT3, NE2TL11 and NE2HP2, were isolated from the inner tissues (leaf and stem) of and roots of . They were characterized using a polyphasic approach, which revealed that they represent two novel species. Phylogenetic analysis based on 16S rRNA gene sequencing showed that the species closest to NE2HP2 was DSM 20754 (99.

Carbonic Anhydrase Regulates Cytoplasmic pH of Nitrogen-Fixing Vesicles.

A phyloprofile of genomes was carried out to identify those genes present in symbiotic strains of clusters 1, 1c, 2 and 3 and absent in non-infective strains of cluster 4. At a threshold of 50% AA identity, 108 genes were retrieved. Among these were known symbiosis-associated genes such as (nitrogenase), and genes which are not know as symbiosis-associated genes such as (carbonic anhydrase, CAN).

Descriptions of nom. nov., comb. nov. and sp. nov. to resolve problems with the taxonomy and nomenclature of strains named .

The name has been used for three different organisms. The first organism with this name is the species represented by strain DCWR9-8-2, a species published in 2015 but whose name was never validated. In 2019 the type species of the genus was reclassified into the genus , while maintaining its original epithet, thus establishing the second group of organisms known as , but the first for which the name was validated.

A Nonspecific Lipid Transfer Protein with Potential Functions in Infection and Nodulation.

The response of to ACN14a is driven by several sequential physiological events from calcium spiking and root-hair deformation to the development of the nodule. Early stages of actinorhizal symbiosis were monitored at the transcriptional level to observe plant host responses to . Forty-two genes were significantly upregulated in inoculated compared with noninoculated roots.

29th Annual GP2A Medicinal Chemistry Conference.

The 29th Annual GPA (Group for the Promotion of Pharmaceutical chemistry in Academia) Conference was a virtual event this year due to the COVID-19 pandemic and spanned three days from Wednesday 25 to Friday 27 August 2021. The meeting brought together an international delegation of researchers with interests in medicinal chemistry and interfacing disciplines. Abstracts of keynote lectures given by the 10 invited speakers, along with those of the 8 young researcher talks and the 50 flash presentation posters, are included in this report.

sp. nov., a novel plant growth-promoting bacterium used in heavy metal polluted soils as a phytoremediation tool.

Strain EAR8 is a root endophyte isolated from plants collected from the Odiel marshes, Huelva (Spain). It presented plant growth-promoting properties and improved the plant growth and heavy metal accumulation in polluted soils playing an important role in phytoremediation strategies. Phenotypically, strain EAR8 cells were Gram-positive, aerobic and non-motile rods with terminal oval endospores and non-swollen sporangia which form beige, opaque, butyrous, raised and irregular colonies with undulate margins.

Analysis of the Interaction between L. and Strains Nodulating This Legume in Northwest Spain.

L. (pea) is one of the most cultivated grain legumes in European countries due to the high protein content of its seeds. Nevertheless, the rhizobial microsymbionts of this legume have been scarcely studied in these countries.

Micromonospora metallophores: A plant growth promotion trait useful for bacterial-assisted phytoremediation?

Heavy metal pollution in the environment is an increasing problem due to natural and anthropogenic activities. The use of bacteria for bioremediation of soils contaminated with heavy metals has gained a lot of attention as it can be considered effective, economic and environmentally sustainable. In this work, we investigated the capacity of endophytic Micromonospora strains isolated from different legumes, to produce metallophores against a variety of heavy metals in vitro.

sp. nov. a novel plant growth-promoting bacterium with potential use in phytoremediation.

Strain RA15 was isolated from the rhizosphere of the halophyte plant growing in the Odiel marshes (Huelva, Spain). RA15 cells were Gram stain-negative, non-spore-forming, aerobic rods and formed cream-coloured, opaque, mucoid, viscous, convex, irregular colonies with an undulate margin. Optimal growth conditions were observed on tryptic soy agar (TSA) plates supplemented with 2.

Recent Progress in the Development of Small-Molecule FtsZ Inhibitors as Chemical Tools for the Development of Novel Antibiotics.

Antibiotics are potent pharmacological weapons against bacterial pathogens, nevertheless their efficacy is becoming compromised due to the worldwide emergence and spread of multidrug-resistant bacteria or "superbugs". Antibiotic resistance is rising to such dangerous levels that the treatment of bacterial infections is becoming a clinical challenge. Therefore, urgent action is needed to develop new generations of antibiotics that will help tackle this increasing and serious public health problem.

Micromonospora orduensis sp. nov., isolated from deep marine sediment.

A novel actinobacterial strain, designated S2509, was isolated from marine sediment collected by a dredge at a depth of 45 m along Melet River offshore of the southern Black Sea coast, Ordu, Turkey. The cell wall peptidoglycan of strain was found to contain meso-diaminopimelic acid and 3-OH-diaminopimelic acid. The whole cell sugars detected were arabinose, glucose, rhamnose, ribose and xylose.

sp. nov., isolated from growing in the Odiel marshes(Spain) and emended descriptions of and .

Strain EAR18 was isolated as an endophyte from the roots of a halophyte plant, , growing in the Odiel marshes (Huelva, Spain). Cells of strain EAR18 were Gram- stain-negative, motile, non-spore-forming aerobic rods. It grew optimally on tryptic soy agar supplemented with 2.

sp. nov., a novel actinobacterium isolated from a high altitude Atacama Desert soil.

A strain, designated 5R2A7, isolated from a high altitude Atacama Desert soil was examined by using a polyphasic approach. Strain 5R2A7 was found to have morphological, chemotaxonomic and cultural characteristics typical of members of the genus The cell wall contains - and -diaminopimelic acid, the major whole-cell sugars are glucose, ribose and xylose, the predominant menaquinones MK-10(H), MK-10(H), MK-10(H) and MK-9(H), the major polar lipids diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol and an unknown glycolipid, and the predominant cellular fatty acids -C, -C and 10-methyl C. The digital genomic DNA G+C content is 72.

Genomic Insights Into Plant-Growth-Promoting Potentialities of the Genus .

This study was designed to determine the plant growth promoting (PGP) potential of members of the genus . To this end, the genomes of 21 representative strains were examined for genes associated directly or indirectly with plant growth. All of the genomes contained genes that encoded for products associated with the biosynthesis of auxins [indole-3-glycerol phosphate synthases, anthranilate phosphoribosyltransferases (D), anthranilate synthases, and aminases (A and B)], cytokinins (11 well-conserved genes within the predicted biosynthetic gene cluster), siderophores, and nitrogenases ( operon except for atypical ) as well as genes that modulate the effects of biotic and abiotic environmental stress (e.

Genomic Insight into Three Marine sp. Strains from the Gulf of California.

Three actinomycete strains, designated BL1, BL4, and CV4, were isolated from sediment samples from the Gulf of California in 2009 together with nearly 300 other actinobacteria. Genome mining and analysis of their ∼6.4-Mb sequences confirmed the bioprospecting potential of these three bacteria belonging to the genus .

Tricoupled hybrid lattice Boltzmann model for nonisothermal drying of colloidal suspensions in micropore structures.

A tricoupled hybrid lattice Boltzmann model (LBM) is developed to simulate colloidal liquid evaporation and colloidal particle deposition during the nonisothermal drying of colloidal suspensions in micropore structures. An entropic multiple-relaxation-time multirange pseudopotential two-phase LBM for isothermal interfacial flow is first coupled to an extended temperature equation for simulating nonisothermal liquid drying. Then the coupled model is further coupled with a modified convection diffusion equation to consider the nonisothermal drying of colloidal suspensions.

Uncovering the potential of novel micromonosporae isolated from an extreme hyper-arid Atacama Desert soil.

The taxonomic status, biotechnological and ecological potential of several Micromonospora strains isolated from an extreme hyper arid Atacama Desert soil were determined. Initially, a polyphasic study was undertaken to clarify the taxonomic status of five micromonosporae, strains LB4, LB19, LB32, LB39 and LB41, isolated from an extreme hyper-arid soil collected from one of the driest regions of the Atacama Desert. All of the isolates were found to have chemotaxonomic, cultural and morphological properties consistent with their classification in the genus Micromonospora.

Jiangella anatolica sp. nov. isolated from coastal lake soil.

A novel actinobacterial strain, designated GTF31, was isolated from a coastal soil sample of Gölcük Lake, a crater lake in southwest Anatolia, Turkey. The taxonomic position of the strain was established using a polyphasic approach. Phylogenetic analyses based on 16S rRNA gene sequences and showed that the strain is closely related to Jiangella gansuensis DSM 44835 (99.

Protein-protein interactions in bacteria: a promising and challenging avenue towards the discovery of new antibiotics.

Antibiotics are potent pharmacological weapons against bacterial infections; however, the growing antibiotic resistance of microorganisms is compromising the efficacy of the currently available pharmacotherapies. Even though antimicrobial resistance is not a new problem, antibiotic development has failed to match the growth of resistant pathogens and hence, it is highly critical to discover new anti-infective drugs with novel mechanisms of action which will help reducing the burden of multidrug-resistant microorganisms. Protein-protein interactions (PPIs) are involved in a myriad of vital cellular processes and have become an attractive target to treat diseases.

Genome-Based Taxonomic Classification of the Phylum .

The application of phylogenetic taxonomic procedures led to improvements in the classification of bacteria assigned to the phylum but even so there remains a need to further clarify relationships within a taxon that encompasses organisms of agricultural, biotechnological, clinical, and ecological importance. Classification of the morphologically diverse bacteria belonging to this large phylum based on a limited number of features has proved to be difficult, not least when taxonomic decisions rested heavily on interpretation of poorly resolved 16S rRNA gene trees. Here, draft genome sequences of a large collection of actinobacterial type strains were used to infer phylogenetic trees from genome-scale data using principles drawn from phylogenetic systematics.